The reconstruction of bone defects by guiding autologous bone tissue regeneration with artificial biomaterials is a potential strategy in the area of bone tissue engineering. The development of new polymers with good biocompatibility, favorable mechanical properties, and osteoinductivity is of vital importance. Graphene and its derivatives have attracted extensive interests due to the exceptional physiochemical and biological properties of graphene. In this study, poly(lactic-co-glycolic acid) (PLGA) films incorporated by graphene nanoplates were fabricated. The results indicated that the incorporation of proper graphene nanoplates into poly(lactic-co-glycolic acid) film could enhance the adhesion and proliferation of rat bone marrow-derived mesenchymal stem cells (rBMSCs). The augmentation of alkaline phosphatase activity, calcium mineral deposition, and the expression level of osteogenic-related genes of rBMSCs on the composite films were observed. Moreover, the incorporation of graphene might activate the PI3K/Akt/GSK-3β/β-catenin signaling pathway, which appeared to be the mechanism behind the osteoinductive properties of graphene. Moreover, the in vivo furcation defect implantation results revealed better guiding bone regeneration properties in the graphene-incorporated group. Thus, we highlight this graphene-incorporated film as a promising platform for the growth and osteogenic differentiation of BMSCs that can achieve application in bone regeneration.

中文翻译：

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聚（乳酸-增强成骨细胞分化和骨再生共-glycolic酸）由石墨烯通过PI3K / AKT / GSK-3β/β连环蛋白信号电路的激活

通过用人造生物材料引导自体骨组织再生来重建骨缺损是骨组织工程领域中的潜在策略。具有良好的生物相容性，良好的机械性能和骨诱导性的新型聚合物的开发至关重要。石墨烯及其衍生物因其优异的理化和生物学特性而引起了广泛的兴趣。在这项研究中，聚（乳酸-共-glycolic乙酸）（PLGA）由石墨烯纳米片并入膜制造。结果表明，适当的石墨烯纳米片并入聚（乳酸-共-乙醇酸膜可以增强大鼠骨髓间充质干细胞（rBMSCs）的粘附和增殖。观察到碱性磷酸酶活性的增强，钙矿物质的沉积以及复合膜上rBMSCs成骨相关基因的表达水平。此外，石墨烯的掺入可能激活PI3K / Akt /GSK-3β/β-catenin信号传导途径，这似乎是石墨烯骨诱导特性的机制。而且，体内分叉缺损植入结果显示，在掺入石墨烯的组中更好的引导骨再生性能。因此，我们重点介绍了这种石墨烯结合膜，它是BMSCs生长和成骨分化的有前途的平台，可以实现骨再生中的应用。