Concerns are growing about human exposure to endocrine disrupting chemicals (EDCs), especially during developmental stages. Parabens (PBs) and ultraviolet filters (UVFs) are prevalent EDCs widely used as additives in cosmetics and personal care products (PCPs). The objective of this study was to develop a method to determine four PBs and ten UVFs in human milk using QuEChERS treatment and ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS). Multivariate strategies were applied to optimize experimental parameters. Limits of quantification ranged from 0.1 to 0.2 ng mL⁻¹ and inter-day variability (evaluated as relative standard deviation) from 6% to 13%. The method was validated using matrix-matched standard calibration followed by a recovery assay with spiked samples. Recovery percentages ranged from 87% to 112%. The method was satisfactorily applied to assess target compounds in human milk samples from 15 donors. This analytical procedure can provide information on newborn exposure to these EDCs.

人们越来越暴露于内分泌干扰化学物质（EDC），尤其是在发育阶段。对羟基苯甲酸酯（PB）和紫外线滤光片（UVF）是普遍的EDC，广泛用作化妆品和个人护理产品（PCP）的添加剂。这项研究的目的是开发一种使用QuEChERS处理和超高效液相色谱-串联质谱法（UHPLC-MS / MS）测定人乳中4种PB和10种UVF的方法。应用多元策略优化实验参数。定量限为0.1到0.2 ng mL ^-1日间波动率（以相对标准偏差评估）为6％至13％。使用基质匹配的标准校准方法，然后对加标样品进行回收率测定，验证了该方法的有效性。回收率从87％到112％不等。该方法令人满意地用于评估15个供体的人乳样品中的目标化合物。该分析程序可以提供有关新生儿接触这些EDC的信息。