As a critical gaseous signaling molecule, H₂S is involved in various biological processes. To deeper study the physiological and pathological roles of H₂S, convenient and efficient detection techniques for endogenous H₂S in vivo are still in urgent demand. Herein, we reported a new turn-on Near-infrared (NIR) fluorescence probe NIR-H₂S based on thiolysis reactions for detection of H₂S. The probe possessed many excellent properties including high sensitivity and selectivity, good cell-membrane permeability, and low cytotoxicity. In vitro, NIR-H₂S showed a 58-fold fluorescence enhancement when reacted with H₂S in a buffer and displayed a good linear relationship (r = 0.9925) in a rather wide concentration range of H₂S (0–500 μM). Furthermore, NIR-H₂S was successfully employed in monitoring endogenous H₂S induced by D-Cys in living cancer cells and mice. These results indicated that NIR-H₂S had great potentiality in detecting cellular H₂S in living animals and being applied to cancer diagnosis.

H ₂ S作为一种重要的气体信号分子，参与了各种生物过程。为了深入研究H ₂ S的生理和病理作用，仍然迫切需要便捷，有效的体内内源H ₂ S检测技术。在本文中，我们报道了新的导通近红外（NIR）荧光探针NIR-H ₂ S的基于硫解反应检测H的₂ S.探针具有许多优良的性能，包括高的灵敏度和选择性，良好的细胞膜渗透性，且细胞毒性低。在体外，NIR-H ₂ S与H ₂反应时显示出58倍的荧光增强S在缓冲液中，并 在相当宽的H ₂ S（0–500μM）浓度范围内表现出良好的线性关系（r = 0.9925 ）。此外，NIR-H ₂ S为在成功地监测内源性ħ采用₂ š诱导d在活癌细胞和小鼠-Cys。这些结果表明，NIR-H ₂ S IN检测蜂窝H具有很大的潜力₂在活的动物和被施加到癌症诊断S上。