With advances in nanotechnology, particles with various size, shape, surface chemistry, and composition can be easily produced. Nano- and microparticles have been extensively explored in many industrial and clinical applications. Ensuring that the particles themselves are not possessing toxic effects to the biological system is of paramount importance. This paper describes a proof of concept method, in which a microfluidic system is used in conjunction with a cell microarray technique aiming to streamline the analysis of particle–cell interaction in a high throughput manner. Polymeric microparticles, with different particle surface functionalities, were first used to investigate the efficiency of particle–cell adhesion under dynamic flow. Silver nanoparticles (AgNPs, 10 nm in diameter) perfused at different concentrations (0 to 20 μg/mL) in parallel streams over the cell microarray exhibited a higher toxicity compared to the static culture in the 96-well-plate format. This developed microfluidic system can be easily scaled up to accommodate a larger number of microchannels for high throughput analysis of the potential toxicity of a wide range of particles in a single experiment.

中文翻译：

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微流控细胞微阵列平台，用于高通量分析颗粒-细胞相互作用

随着纳米技术的进步，可以容易地生产具有各种尺寸，形状，表面化学性质和组成的颗粒。纳米和微粒已在许多工业和临床应用中得到了广泛的探索。确保颗粒本身对生物系统不具有毒性作用至关重要。本文介绍了一种概念验证方法，其中将微流体系统与细胞微阵列技术结合使用，旨在以高通量的方式简化对粒子-细胞相互作用的分析。具有不同颗粒表面功能的聚合物微粒首先用于研究动态流动下颗粒与细胞之间的粘附效率。银纳米颗粒（AgNPs，与96孔板形式的静态培养物相比，以不同浓度（0至20μg/ mL）在细胞微阵列上以平行流灌注的直径10 nm）显示出更高的毒性。这种发达的微流控系统可以轻松扩展规模，以容纳大量的微通道，从而可以在单个实验中对大量颗粒的潜在毒性进行高通量分析。