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Accumulation and effects of copper on aquatic macrophytes Potamogeton pectinatus L.: Potential application to environmental monitoring and phytoremediation
Ecotoxicology and Environmental Safety ( IF 6.8 ) Pub Date : 2018-03-03 , DOI: 10.1016/j.ecoenv.2018.01.062
Marcela Brandão Costa , Francesca Valêncio Tavares , Claudia Bueno Martinez , Ioni Gonçalves Colares , Camila de Martinez Gaspar Martins

This study investigated the ability of Potamogeton pectinatus L. to accumulate copper and its effects on plants. In accumulation tests, macrophytes were exposed (96 h) to different copper concentrations (0–1000 µM) and the metal was measured in media and plant tissues (roots, stems and leaves) to determine the bioconcentration factor (BCF). Plants accumulated high concentrations of copper in a dose-dependent manner and roots was the main organ for copper accumulation. However, the more copper increased in water, the more BCF values decreased. It may be due to either saturation of copper uptake or down-regulation of metal uptake by plants. In the physiological and morphological analyses, plants were kept (96 h) in Hoagland nutrient solution without copper, in full Hoagland solution (0.5 µM Cu) and in Hoagland medium with copper from 1 to 100 µM. The absence and the presence of copper above to 1 µM inhibited photosynthesis. Chlorophylls and carotenoid levels also decreased with the excess of copper, a fact that may have affected the photosystem II-dependent of chlorophyll and caused photosynthesis suppression. Only macrophytes at 10 µM Cu showed decrease in length and number of leaves on the 10th day of the test, when they died. Chlorosis and necrosis were observed in control groups and groups with extra copper, but not in Hoalgand group. Overall, the macrophyte P. pectinatus can be considered a suitable plant for monitoring environments contaminated by copper, based on results of copper accumulation in the plant, decrease in pigment concentration and presence of chlorosis and necrosis. However, values of BCF based on fresh water tissues was not proper to indicate the use of P. pectinatus for cleaning environments contaminated by copper.



中文翻译:

铜在水生植物Potamogeton pectinatus L.上的富集及其影响:在环境监测和植物修复中的潜在应用

这项研究调查了Potamogeton pectinatus的能力L.积累铜及其对植物的影响。在积累测试中,大型植物被暴露(96小时)于不同的铜浓度(0–1000 µM)中,并在培养基和植物组织(根,茎和叶)中测量了金属含量,以确定生物富集系数(BCF)。植物以剂量依赖的方式积累高浓度的铜,而根是铜积累的主要器官。但是,水中铜的增加越多,BCF值下降的越多。这可能是由于铜吸收饱和或植物对金属吸收的下调所致。在生理和形态分析中,将植物分别在无铜的Hoagland营养液,全Hoagland溶液(0.5 µM Cu)和含1至100 µM铜的Hoagland培养基中保存(96小时)。铜的存在和超过1 µM都抑制了光合作用。叶绿素和类胡萝卜素水平也随着铜的过量而降低,这一事实可能影响了依赖光系统II的叶绿素并导致了光合作用受到抑制。在试验的第10天,只有10 µM Cu的大型植物才死亡,其长度和叶片数量均减少。对照组和含铜过多的组出现黄化和坏死,而霍格兰德组则未见。总体而言,大型植物 对照组和含铜过多的组出现黄化和坏死,而霍格兰德组则未见。总体而言,大型植物 对照组和含铜过多的组出现黄化和坏死,而霍格兰德组则未见。总体而言,大型植物基于植物中铜的积累,色素浓度降低以及褪绿和坏死的存在的结果,可以认为果蝇P. pectinatus被认为是监测铜污染环境的合适植物。但是,基于淡水组织的BCF值并不适合指示将果胶对虾用于清洁被铜污染的环境。

更新日期:2018-03-03
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