The fate of organic chemicals and their metabolites in soils is often investigated in model matrices having undergone various pre-treatment steps that may qualitatively or quantitatively interfere with the results. Presently, effects associated with soil sterilization by γ-irradiation and soil spiking using an organic solvent were studied in one freshly spiked soil (sterilization prior to contamination) and its field-contaminated (sterilization after contamination) counterpart for the model organic compound 1,1-Dichloro-2,2-bis(4-chlorophenyl)ethene (p,p′-DDE). Changes in the sorption and potential bioavailability of spiked and native p,p′-DDE were measured by supercritical fluid extraction (SFE), XAD-assisted extraction (XAD), and solid-phase microextraction (SPME) and linked to qualitative changes in soil organic matter (SOM) chemistry measured by diffuse reflectance infrared Fourier-transform (DRIFT) spectroscopy. Reduced sorption of p,p´-DDE detected with XAD and SPME was associated more clearly with spiking than with sterilization, but SFE showed a negligible impact. Spiking resulted in an increase of the DRIFT-derived hydrophobicity index, but irradiation did not. Spectral peak height ratio descriptors indicated increasing hydrophobicity and hydrophilicity in pristine soil following sterilization, and a greater reduction of hydrophobic over hydrophilic groups as a consequence of spiking. In parallel, reduced sorption of p,p´-DDE upon spiking was observed. Based on the present samples, γ-irradiation appears to alter soil sorptive properties to a lesser extent when compared to common laboratory processes such as spiking with organic solvents.

通常在经过各种预处理步骤（可能定性或定量地干扰结果）的模型矩阵中研究土壤中有机化学物及其代谢产物的命运。目前，在一种新加标的土壤中（污染前灭菌）及其与模型有机化合物1,1对应的被现场污染（污染后灭菌）的土壤中，研究了通过γ射线辐射和使用有机溶剂加标土壤的杀菌作用。 -二氯-2，2-双（4-氯苯基）乙烯（p， p′-DDE）。尖峰和天然p，p的吸附和潜在生物利用度的变化通过超临界流体萃取（SFE），XAD辅助萃取（XAD）和固相微萃取（SPME）来测量'-DDE，并通过漫反射红外傅里叶变换测量土壤有机质（SOM）化学的质性变化。 （DRIFT）光谱。用XAD和SPME检测到的对p，p´- DDE吸附减少与加标作用明显比与灭菌作用有关，但SFE的影响可忽略不计。掺入会导致DRIFT衍生的疏水性指数增加，但辐照却没有。光谱峰高比描述符表明灭菌后原始土壤中的疏水性和亲水性增加，并且由于尖峰的作用，疏水性比亲水性基团有更大的降低。同时，减少吸附观察到尖峰时的p，p´- DDE。根据目前的样品，与普通的实验室方法（例如用有机溶剂加标）相比，γ辐照似乎在较小程度上改变了土壤的吸附特性。