A number of tools based on high-performance affinity separations have been developed for studying drug-protein interactions. An example of one recent approach is ultrafast affinity extraction. This method has been employed to examine the free (or non-bound) fractions of drugs and other solutes in simple or complex samples that contain soluble binding agents. These free fractions have also been used to determine the binding constants and rate constants for the interactions of drugs with these soluble agents. This report describes the general principles of ultrafast affinity extraction and the experimental conditions under which it can be used to characterize such interactions. This method will be illustrated by utilizing data that have been obtained when using this approach to measure the binding and dissociation of various drugs with the serum transport proteins human serum albumin and alpha1-acid glycoprotein. A number of practical factors will be discussed that should be considered in the design and optimization of this approach for use with single-column or multi-column systems. Techniques will also be described for analyzing the resulting data for the determination of free fractions, rate constants and binding constants. In addition, the extension of this method to complex samples, such as clinical specimens, will be considered.

中文翻译：

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通过超快亲和萃取表征溶液相药物-蛋白质相互作用

已经开发了许多基于高性能亲和分离的工具来研究药物-蛋白质相互作用。最近一种方法的一个例子是超快亲和力提取。该方法已被用于检查含有可溶性结合剂的简单或复杂样品中药物和其他溶质的游离（或非结合）部分。这些游离部分也已用于确定药物与这些可​​溶性药物相互作用的结合常数和速率常数。本报告描述了超快亲和力提取的一般原理以及可用于表征此类相互作用的实验条件。该方法将利用在使用这种方法测量各种药物与血清转运蛋白、人血清白蛋白和 alpha1-酸性糖蛋白的结合和解离时获得的数据来说明。将讨论在设计和优化此方法以用于单塔或多塔系统时应考虑的许多实际因素。还将描述用于分析所得数据以确定游离部分、速率常数和结合常数的技术。此外，还将考虑将此方法扩展到复杂样本，例如临床标本。将讨论在设计和优化此方法以用于单塔或多塔系统时应考虑的许多实际因素。还将描述用于分析所得数据以确定游离部分、速率常数和结合常数的技术。此外，还将考虑将此方法扩展到复杂样本，例如临床标本。将讨论在设计和优化此方法以用于单塔或多塔系统时应考虑的许多实际因素。还将描述用于分析所得数据以确定游离部分、速率常数和结合常数的技术。此外，还将考虑将此方法扩展到复杂样本，例如临床标本。