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Data‐Matrix Technology for Multiparameter Monitoring of Cell Cultures
Small Methods ( IF 12.4 ) Pub Date : 2018-03-05 , DOI: 10.1002/smtd.201700377
Marianna Barbalinardo 1 , Denis Gentili 1 , Francesca Lazzarotto 1 , Francesco Valle 1 , Marco Brucale 1 , Manuela Melucci 2 , Laura Favaretto 2 , Massimo Zambianchi 2 , Ana I. Borrachero-Conejo 1 , Emanuela Saracino 2 , Valentina Benfenati 2 , Davide Natalini 3 , Paolo Greco 3 , Maria Giovanna Di Carlo 3 , Giulia Foschi 3 , Massimiliano Cavallini 1
Affiliation  

A data‐matrix (DM)‐technology approach in cell biology is implemented as an efficient method for the multiparameter monitoring of cell cultures. The proposed method takes advantage of the know‐how developed for fault tolerance in digital information technology by measuring the amount of errors induced by intervening cells upon checking a DM code placed behind them. It gives continuous access to several quantitative parameters of the observed culture, such as cell coverage, mean size, viability, and transfection efficiency.

中文翻译:

用于细胞培养多参数监测的数据矩阵技术

细胞生物学中的数据矩阵(DM)技术方法是一种有效的细胞培养多参数监测方法。所提出的方法利用数字信息技术中为容错而开发的专有技术,通过在检查放置在其后的DM代码后测量居间单元引起的错误量,来利用这些知识。它可以连续访问观察到的培养物的几个定量参数,例如细胞覆盖率,平均大小,生存力和转染效率。
更新日期:2018-03-05
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