Development of an efficient method for the simultaneous detection of two highly concerning mycotoxins, ochratoxin A (OTA) and aflatoxin B1 (AFB1), is of great significance on food safety monitoring. Herein, a magnetically controlled fluorescence aptasensor for simultaneous determination of OTA and AFB1 has been successfully developed. The working principle of the aptasensor is based on the specific aptamer-mycotoxin recognition and further leads to the partial release of two distinguishable fluorescence labels from the magnetic carriers. Through the magnetic separation, the reporter probes in the supernatant solution can be collected and converted into a sensitive fluorescence signal with dual emission peaks. This aptasensor provided a wide detection range of 2 pg mL-1 - 5 ng mL-1 for OTA and 5 pg mL-1 - 10 ng mL-1 for AFB1. The new easy-to-wash and simple-to-use approach offers a simultaneous and high selective detection with high sensitivity (limits of detection of 0.67 and 1.70 pg mL-1 for OTA and AFB1, respectively). Remarkable accuracy (relative standard deviation < 5.6%) during the mycotoxins determination as well as excellent quantitative recoveries (95-108%) during the analysis of the spiked corn samples were also achieved. This simple aptasensing scheme provides a new avenue for high throughput screen of dual mycotoxins due to its simple manipulation, short assay times, high selectivity and sensitivity.

中文翻译：

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用于同时测定赭曲霉毒素 A 和黄曲霉毒素 B1 的磁控荧光适体传感器

开发一种同时检测两种高度关注的真菌毒素赭曲霉毒素 A (OTA) 和黄曲霉毒素 B1 (AFB1) 的有效方法对食品安全监测具有重要意义。在此，成功开发了一种用于同时测定 OTA 和 AFB1 的磁控荧光适体传感器。适体传感器的工作原理基于特异性适体-霉菌毒素识别，并进一步导致从磁性载体中部分释放两种可区分的荧光标记。通过磁分离，可以收集上清液中的报告探针，并将其转化为具有双发射峰的灵敏荧光信号。该适体传感器为 OTA 提供了 2 pg mL-1 - 5 ng mL-1 的检测范围，为 AFB1 提供了 5 pg mL-1 - 10 ng mL-1 的检测范围。新的易于清洗和易于使用的方法提供了高灵敏度的同步和高选择性检测（OTA 和 AFB1 的检测限分别为 0.67 和 1.70 pg mL-1）。在霉菌毒素测定过程中也实现了显着的准确度（相对标准偏差 < 5.6%），在加标玉米样品的分析过程中也实现了出色的定量回收率 (95-108%)。由于操作简单、检测时间短、选择性和灵敏度高，这种简单的适体传感方案为双真菌毒素的高通量筛选提供了新途径。6%) 在霉菌毒素测定过程中以及在分析加标玉米样品期间实现了出色的定量回收率 (95-108%)。由于操作简单、检测时间短、选择性和灵敏度高，这种简单的适体传感方案为双真菌毒素的高通量筛选提供了新途径。6%) 在霉菌毒素测定过程中以及在分析加标玉米样品期间实现了出色的定量回收率 (95-108%)。由于操作简单、检测时间短、选择性和灵敏度高，这种简单的适体传感方案为双真菌毒素的高通量筛选提供了新途径。