Capillary electrophoresis (CE) is an effective tool for protein separation and analysis. Compared with capillary gel electrophoresis (CGE), non-gel sieving capillary electrophoresis (NGSCE) processes the superiority on operation, repeatability and automaticity. Herein, we investigated the effect of polymer molecular weight and concentration, electric field strength, and the effective length of the capillary on the separation performance of proteins, and find that (1) polymer with high molecular weight and concentration favors the separation of proteins, although concentrated polymer hinders its injection into the channel of the capillary due to its high viscosity. (2) The resolution between the adjacent proteins decreases with the increase of electric field strength. (3) When the effective length of the capillary is long, the separation performance improves at the cost of separation time. (4) 1.4% (w/v) hydroxyethyl cellulose (HEC), 100 V/cm voltage and 12 cm effective length offers the best separation for the proteins with molecular weight from 14,400 Da to 97,400 Da. Finally, we employed the optimal electrophoretic conditions to resolve Lysozyme, Ovalbumin, BSA and their mixtures, and found that they were baseline resolved within 15 min.

毛细管电泳（CE）是蛋白质分离和分析的有效工具。与毛细管凝胶电泳（CGE）相比，非凝胶筛分毛细管电泳（NGSCE）在操作，重复性和自动化方面具有优越性。在此，我们研究了聚合物分子量和浓度，电场强度以及毛细管的有效长度对蛋白质分离性能的影响，发现（1）高分子量和高浓度的聚合物有利于蛋白质的分离，尽管浓缩的聚合物由于其高粘度而阻止其注入毛细管通道。（2）相邻蛋白质之间的分辨率随着电场强度的增加而降低。（3）当毛细管的有效长度较长时，分离性能的提高以分离时间为代价。（4）1.4％（w / v）羟乙基纤维素（HEC），100 V / cm电压和12 cm有效长度为分子量从14,400 Da至97,400 Da的蛋白质提供了最佳分离效果。最后，我们采用最佳的电泳条件来解析溶菌酶，卵清蛋白，BSA及其混合物，并发现它们在15分钟内被基线分离。