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Single-molecule analysis of microRNA and logic operations using a smart plasmonic nanobiosensor
Journal of the American Chemical Society ( IF 15.0 ) Pub Date : 2018-03-05 , DOI: 10.1021/jacs.7b12772
Ying Zhang 1 , Zhenhua Shuai 1 , Hao Zhou 1 , Zhimin Luo 1 , Bing Liu 2 , Yinan Zhang 2 , Lei Zhang 1 , Shufen Chen 1 , Jie Chao 1 , Lixing Weng 1 , Quli Fan 1 , Chunhai Fan 1, 2 , Wei Huang 1, 3 , Lianhui Wang 1
Affiliation  

Analysis of biomolecules at the single-molecule level is a great challenge in molecular diagnostics, gene profiling, and environmental monitoring. In this work, we design a smart plasmonic nanobiosensor based on individual Au@Ag core-shell nanocube (Au@Ag NC) modified with tetrahedron-structured DNA (tsDNA) for detecting microRNA 21 (miR-21) at the single-molecule level. An average localized surface plasmon resonance (LSPR) scattering spectral wavelength shift of approximately 0.4 nm can be obtained for a single miR-21 hybridization event on the nanobiosensor. In addition, the sensing mechanism of the individual Au@Ag NC is further verified by the three-dimensional finite-difference time-domain (3D-FDTD) simulations. Notably, this system not only allows the real-time detection of miR-21 with an aM level sensitivity over a large dynamic range from 1 aM to 1 nM, but also enables DNA-based logic operations as well as biomemory by exploiting miR-21, KpnI, and StuI-responsive assays. Our study opens a unique method for single-molecule detection of biomolecules and thus holds great promise in a variety of biological and biomedical applications.

中文翻译:

使用智能等离子体纳米生物传感器对 microRNA 和逻辑运算进行单分子分析

单分子水平的生物分子分析是分子诊断、基因分析和环境监测的一大挑战。在这项工作中,我们设计了一种基于用四面体结构 DNA (tsDNA) 修饰的单个 Au@Ag 核壳纳米立方体 (Au@Ag NC) 的智能等离子体纳米生物传感器,用于在单分子水平检测 microRNA 21 (miR-21) . 对于纳米生物传感器上的单个 miR-21 杂交事件,可以获得大约 0.4 nm 的平均局部表面等离子体共振 (LSPR) 散射光谱波长偏移。此外,通过三维有限差分时域 (3D-FDTD) 模拟进一步验证了单个 Au@Ag NC 的传感机制。尤其,该系统不仅可以在 1 aM 到 1 nM 的大动态范围内实时检测具有 aM 级别灵敏度的 miR-21,而且还可以通过利用 miR-21、KpnI 实现基于 DNA 的逻辑操作以及生物记忆和 StuI 反应性测定。我们的研究为生物分子的单分子检测开辟了一种独特的方法,因此在各种生物和生物医学应用中具有广阔的前景。
更新日期:2018-03-05
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