Acetyl coenzyme A is involved in several key metabolic pathways. Its concentration can vary considerably in response to physiological or pathological conditions making it a potentially valuable biomarker. However, little information about the measurement and concentration of acetyl CoA in human whole blood is found in the literature. The aim of this study was the development of an accurate method for the determination of acetyl CoA in human whole blood by LC–MS/MS. The method, involving extraction from whole blood by a rapid protein precipitation procedure was thoroughly validated: limit of quantitation was 3.91 ng mL⁻¹. Accuracy and precision were calculated at five concentrations and were within ±15%.

The average endogenous level of acetyl CoA in human whole blood was determined in 17 healthy individuals to be 220.9 ng mL⁻¹ (ranging from 124.0 to 308.0 ng mL⁻¹). This represents, to our knowledge, the first report of acetyl CoA levels in human whole blood, and the first practical and reliable method for its determination.

乙酰辅酶A参与几种关键的代谢途径。其浓度可响应于生理或病理状况而显着变化，使其成为潜在有价值的生物标志物。然而，在文献中几乎没有关于人全血中乙酰辅酶A的测量和浓度的信息。这项研究的目的是开发一种通过LC-MS / MS测定人全血中乙酰辅酶A的准确方法。通过快速蛋白质沉淀程序从全血中提取的方法得到了充分验证：定量限为3.91 ng mL ^-1。在五个浓度下计算出的准确度和精密度均在±15％之内。

在17位健康个体中，人全血中乙酰CoA的平均内源性水平确定为220.9 ng mL ^-1（范围从124.0到308.0 ng mL ^-1）。据我们所知，这是人类全血中乙酰辅酶A水平的第一份报告，也是测定它的第一种实用且可靠的方法。