There are few data on the effects of methotrexate on reproductive capacity in men with inflammatory bowel diseases (IBDs). We performed a case-control study to determine the effects of methotrexate on sperm quality and genetic integrity. We compared sperm samples from 7 men with IBD who had been exposed to methotrexate for at least 3 months with sperm samples collected from 1912 age-matched men at fertility centers (controls) where sperm parameters would be expected to be worse than those of the general population. Sperm were evaluated by basic semen analysis and advanced sperm integrity testing. In samples from men with IBD, all basic semen analysis parameters were within normal limits. However, these samples had reduced sperm integrity, based on significant increases in levels of DNA fragmentation and damage from oxidative stress compared with controls. Our findings indicate that methotrexate can reduce DNA integrity in sperm and cause damage via oxidative stress.

关于甲氨蝶呤对炎症性肠病（IBDs）男性生殖能力影响的数据很少。我们进行了一项病例对照研究，以确定甲氨蝶呤对精子质量和遗传完整性的影响。我们将7名曾接受甲氨蝶呤暴露至少3个月的IBD男性的精子样本与1912例年龄匹配的男性在生育中心（对照）收集的精子样本进行了比较，这些人的精子参数可能比一般人的精子参数差。人口。通过基本精液分析和高级精子完整性测试评估精子。在患有IBD的男性样本中，所有基本的精液分析参数均在正常范围内。但是，这些样品降低了精子的完整性，与对照组相比，其基于DNA片段化水平和氧化应激损伤的显着增加。我们的研究结果表明，甲氨蝶呤可以降低精子中的DNA完整性，并通过氧化应激引起损害。