Protein interactions with specific nucleic acid sequences are crucial in cell growth. Inspired by such binding events that often occur at nanoscale biointerface, here a trans‐scale functional interface capable of considerably enhancing in vitro DNA‐enzyme interaction is reported. Using a screen‐printed electrode with nanoroughened carbon surface, the high‐curvature gold nanostructures in a single electrodeposition step can be programmed. In this process, a synergistic effect is found between nanoroughened carbon and polyelectrolyte multilayer enabling the formation of high‐stability and high‐curvature nanostructures. More importantly, these fractal nanostructures effectively overcome neighboring probes aggregation at high density and allow the probes to be more freely accessed by target molecules. As compared to its planar counterparts, this nanostructuring interface demonstrates faster enzymatic dynamics that enables ultrasensitive detection of microRNA with a detection limit of 35 × 10⁻¹⁸ m. Such an efficient trans‐scale biosensing interface has also accurately differentiated the patients with rheumatic arthritis from the health ones, signifying its great potential in precision medicine.

中文翻译：

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生物启发的跨尺度功能接口，可增强酶动力学和微RNA的超灵敏检测

蛋白质与特定核酸序列的相互作用在细胞生长中至关重要。受此类结合事件的启发，这种结合事件通常发生在纳米级生物界面上，据报道，这种跨尺度功能界面能够显着增强体外DNA-酶的相互作用。使用具有纳米粗糙化碳表面的丝网印刷电极，可以对单个电沉积步骤中的高曲率金纳米结构进行编程。在此过程中，在纳米粗糙化的碳和聚电解质多层膜之间发现了协同效应，从而可以形成高稳定性和高曲率的纳米结构。更重要的是，这些分形纳米结构有效地克服了相邻探针在高密度下的聚集，并使探针更易于被目标分子接近。与平面同类产品相比，⁻¹⁸ m。这种有效的跨尺度生物传感界面还可以准确地区分风湿性关节炎患者与健康风湿性关节炎患者，这表明其在精密医学领域的巨大潜力。