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Plasmonic Sensor Could Enable Label-Free DNA Sequencing
ACS Sensors ( IF 8.9 ) Pub Date : 2018-02-28 00:00:00 , DOI: 10.1021/acssensors.7b00957
Arif E. Cetin 1 , Pinar Iyidogan 1 , Yuki Hayashi 1 , Mark Wallen 1 , Kandaswamy Vijayan 1 , Eugene Tu 1 , Michael Nguyen 1 , Arnold Oliphant 1
Affiliation  

We demonstrated a proof-of-principle concept of a label-free platform that enables nucleic acid sequencing by binding methodology. The system utilizes gold surfaces having high fidelity plasmonic nanohole arrays which are very sensitive to minute changes of local refractive indices. Our novel surface chemistry approach ensures accurate identification of correct bases at individual positions along a targeted DNA sequence on the gold surface. Binding of the correct base on the gold sensing surface triggers strong spectral variations within the nanohole optical response, which provides a high signal-to-noise ratio and accurate sequence data. Integrating our label-free sequencing platform with a lens-free imaging-based device, we reliably determined targeted DNA sequences by monitoring the changes within the plasmonic diffraction images. Consequently, this new label-free surface chemistry technique, integrated with plasmonic lens-free imaging platform, will enable monitoring multiple biomolecular binding events, which could initiate new avenues for high-throughput nucleic acid sequencing.

中文翻译:

等离子体传感器可实现无标记DNA测序

我们展示了无标签平台的原理验证概念,该平台可通过结合方法进行核酸测序。该系统利用具有高保真等离子体纳米孔阵列的金表面,该阵列对局部折射率的微小变化非常敏感。我们新颖的表面化学方法可确保准确识别沿金表面上靶向DNA序列的各个位置的正确碱基。在金感测表面上结合正确的碱基会触发纳米孔光学响应内的强烈光谱变化,从而提供高信噪比和准确的序列数据。将我们的无标签测序平台与基于无透镜成像的设备集成在一起,我们通过监控等离子衍射图像中的变化来可靠地确定了目标DNA序列。所以,
更新日期:2018-02-28
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