This paper describes a new approach for the massive production of electrochemical paper-based analytical devices (ePADs). These devices are fully fabricated by screen-printing technology and consist of a lineal microfluidic channel delimited by hydrophobic walls (patterned with diluted ultraviolet screen-printing ink in chromatographic paper grade 4) and a three-electrode system (printed with carbon and/or Ag/AgCl conductive inks). The printing process was characterised and optimized for pattern each layer with only one squeeze sweep. These ePADs were used as transducers to develop a glucose biosensor. Ionic strength/pH buffering salts, electrochemical mediator (ferricyanide) and enzyme (glucose dehydrogenase FAD-dependent) were separately stored along the microfluidic channel in order to be successively dissolved and mixed after the sample dropping at the entrance. The analyses required only 10 µl and the biosensors showed good reproducibility (RSD = 6.2%, n = 10) and sensitivity (0.426 C/M cm²), wide linear range (0.5–50 mM; r² = 0.999) and low limit of detection (0.33 mM). Furthermore, the new biosensor was applied for glucose determination in five commercial soft-drinks without any sample treatment before the analysis. These samples were also analysed with a commercial enzymatic-kit assay. The results indicated that both methods provide accurate results.

本文介绍了一种大规模生产基于电化学纸的分析设​​备（ePAD）的新方法。这些设备是完全通过丝网印刷技术制造的，包括由疏水壁界定的线性微流体通道（在4级色谱纸上用稀释的紫外线丝网印刷油墨进行图案化）和三电极系统（在碳和/或Ag上印刷） / AgCl导电油墨）。对印刷过程进行了表征，并优化了印刷过程，仅需一次挤压就可以对每一层进行构图。这些ePAD被用作开发葡萄糖生物传感器的传感器。离子强度/ pH缓冲盐，沿微流体通道分别存储电化学介质（铁氰化物）和酶（葡萄糖脱氢酶FAD依赖性），以便在样品滴落到入口后依次溶解和混合。分析仅需10 µl，生物传感器显示出良好的重现性（RSD = 6.2％，n = 10）和灵敏度（0.426 C / M cm²），宽线性范围（0.5–50 mM； r ² = 0.999）和低检测限（0.33 mM）。此外，在分析之前，这种新型生物传感器可用于五种市售软饮料中的葡萄糖测定，而无需任何样品处理。还使用商业酶试剂盒分析法对这些样品进行了分析。结果表明，两种方法均能提供准确的结果。