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Discovery of Shoot Branching Regulator Targeting Strigolactone Receptor DWARF14
ACS Central Science ( IF 18.2 ) Pub Date : 2018-02-07 00:00:00 , DOI: 10.1021/acscentsci.7b00554
Masahiko Yoshimura 1 , Ayato Sato , Keiko Kuwata , Yoshiaki Inukai , Toshinori Kinoshita 1 , Kenichiro Itami 1 , Yuichiro Tsuchiya , Shinya Hagihara 1, 2
Affiliation  

DWARF14 (D14) is a strigolactone receptor that plays a central role in suppression of shoot branching, and hence is a potential target to increase crop productions and biomass. Recently, we reported a fluorescence turn-on probe, Yoshimulactone Green (YLG), which generates a strong fluorescence upon the hydrolysis by D14-type strigolactone receptors. Herein, we applied a YLG-based in vitro assay to a high-throughput chemical screening and identified a novel small molecule DL1 as a potent inhibitor of D14. DL1 competes with endogenous strigolactones, thereby increasing the number of shoot branching in a model plant Arabidopsis as well as in rice. Thus, DL1 is expected to be useful not only as a tool to understand the biological roles of D14 receptors in plant growth and development, but also as a potent agrochemical to improve the crop yield.

中文翻译:

发现靶向Strigolactone受体DWARF14的枝条分支调节剂

DWARF14(D14)是strigolactone受体,在抑制枝条的分支中起着核心作用,因此是增加作物产量和生物量的潜在目标。最近,我们报道了一种荧光开启探针Yoshimulactone Green(YLG),该探针在被D14型strigolactone受体水解后会产生强烈的荧光。在这里,我们将基于YLG的体外分析应用于高通量化学筛选,并确定了一种新型的小分子DL1作为D14的有效抑制剂。DL1与内源性strigolactones竞争,从而增加了拟南芥模型植物中枝条的分支数量以及米饭中 因此,预期DL1不仅可用作了解D14受体在植物生长和发育中的生物学作用的工具,而且可用作提高农作物产量的有效农用化学品。
更新日期:2018-02-07
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