In this study, a reduced graphene oxide (rGO)-based nanostructured composite working electrode of high quality was successfully microfabricated and micro-patterned on a flexible polyimide substrate using simple low-cost fabrication processes. Gold and platinum alloy nanoparticles were electrochemically deposited onto the microfabricated rGO surface and chitosan-glucose oxidase composites were integrated onto the modified surface of the working electrode to develop a human sweat-based wearable glucose sensor application. The fabricated biosensor exhibited excellent amperometric response to glucose at a detection range of 0–2.4 mM (covers the glucose range in sweat), with a sensitivity of 48 μA/mMcm², a short response time (20 s), and high linearity (0.99). The detection limit for glucose was calculated as 5 µm. The human sweat/mixing glucose samples initially used for testing indicated acceptable detection performance and stability for low glucose concentrations. These results confirm that the proposed nanostructured composite flexible working electrode and fabrication process are highly promising for application as human sweat-based electrochemical glucose sensors.

在这项研究中，使用简单的低成本制造工艺，成功地在柔性聚酰亚胺基板上成功地微制造了微型化的微型化氧化石墨烯（rGO）基纳米结构复合工作电极。将金和铂合金纳米颗粒电化学沉积到微细加工的rGO表面上，并将壳聚糖-葡萄糖氧化酶复合材料整合到工作电极的修饰表面上，以开发基于汗液的可穿戴式葡萄糖传感器应用。制成的生物传感器在0-2.4 mM的检测范围内表现出对葡萄糖的出色安培响应（覆盖汗液中的葡萄糖范围），灵敏度为48μA/ mMcm ²，响应时间短（20 s）和高线性度（0.99）。葡萄糖的检出限为5μm。最初用于测试的人类汗液/混合葡萄糖样品显示出可接受的检测性能和低葡萄糖浓度的稳定性。这些结果证实了所提出的纳米结构复合柔性工作电极和制造工艺对于作为基于人类汗液的电化学葡萄糖传感器的应用是非常有前途的。