当前位置: X-MOL 学术Nat. Methods › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A hybridization-chain-reaction-based method for amplifying immunosignals
Nature Methods ( IF 48.0 ) Pub Date : 2018-02-26 , DOI: 10.1038/nmeth.4611
Rui Lin , Qiru Feng , Peng Li , Ping Zhou , Ruiyu Wang , Zhe Liu , Zhiqiang Wang , Xiangbing Qi , Nan Tang , Feng Shao , Minmin Luo

Immunosignal hybridization chain reaction (isHCR) combines antibody–antigen interactions with hybridization chain reaction (HCR) technology, which results in amplification of immunofluorescence signals by up to two to three orders of magnitude with low background. isHCR's highly modular and easily adaptable design enables the technique to be applied broadly, and we further optimized its use in multiplexed imaging and in state-of-the-art tissue expansion and clearing techniques.



中文翻译:

基于杂交链反应的免疫信号放大方法

免疫信号杂交链反应(isHCR)将抗体-抗原相互作用与杂交链反应(HCR)技术结合在一起,可在低背景下将免疫荧光信号放大多达2-3个数量级。isHCR的高度模块化和易于适应的设计使该技术得以广泛应用,并且我们进一步优化了其在多路成像以及最新的组织扩张和清除技术中的使用。

更新日期:2018-02-27
down
wechat
bug