The determination and speciation of arsenic in natural resources such as drinking water and agricultural soils has been a growing concern in recent years due to its many toxicological effects [1–3]. To speciate and quantitate concentrations of <1 ppm of arsenic, typically an ion chromatograph (IC) interfaced to an inductively coupled plasma mass spectrometer (ICP-MS) is employed [4–9]. This methodology may be very robust and sensitive, but it is expensive and not as ubiquitous as high performance liquid chromatography (HPLC) with ultraviolet (UV) absorbance detection or electrospray ionization mass spectrometry (ESI-MS). Anion exchange chromatography is a well-documented means of speciating arsenite (As(III), As₂O₃) and arsenate (As(V), AsO₄) using UV [10], conductivity [11], or ESI-MS detection [12,13]. This paper demonstrates the utilization of common liquid chromatographic instrumentation to speciate and determines inorganic Arsenic compounds using UV or MS via selected ion recording (SIR) or multiple reaction monitoring (MRM) detection. This paper describes the analysis of arsenite and arsenate samples prepared using both deionized and ground water. The limit of quantitation for the techniques described in this paper for samples spiked in ground water were 454 ppb (As(III)) and 562 ppb (As(V)) for UV detection, 45.4 ppb (As(III)) and 56.2 ppb (As(V)) for SIR detection, and 4.54 ppb (As(III)) and 5.62 ppb (As(V)) for MRM detection.

近年来，由于饮用水的多种毒理作用，砷在饮用水和农业土壤等自然资源中的确定和形态形成引起了越来越多的关注[1-3]。为了确定和定量浓度小于1 ppm的砷，通常使用与电感耦合等离子体质谱仪（ICP-MS）相连的离子色谱仪（IC）[4-9]。该方法可能非常健壮和灵敏，但价格昂贵，而且不如具有紫外（UV）吸收检测或电喷雾电离质谱（ESI-MS）的高效液相色谱（HPLC）普及。阴离子交换色谱是证明亚砷酸盐（As（III），As ₂ O ₃）和砷酸盐（As（V），AsO ₄），使用紫外线[10]，电导率[11]或ESI-MS检测[12,13]。本文展示了利用普通液相色谱仪器进行形态分析并使用UV或MS通过选定的离子记录（SIR）或多反应监测（MRM）检测来测定无机砷化合物的方法。本文介绍了使用去离子水和地下水制备的砷和砷酸盐样品的分析方法。本文介绍的用于在地下水中加标的样品的技术的定量限值分别为454 ppb（As（III））和562 ppb（As（V））用于紫外线检测，45.4 ppb（As（III））和56.2 ppb （As（V））用于SIR检测，4.54 ppb（As（III））和5.62 ppb（As（V））用于MRM检测。