Poly(lactide-co-glycolide) (PLGA) is one of the most attractive biodegradable polymers for loading and delivering payloads, especially in the form of nanoparticles (NPs). In this work, NPs of PLGA with 3 different molecular weights were fabricated using bovine serum albumin (BSA) and polyethyleneimine (PEI) as dispersing agents. Elemental analysis revealed that the loading amounts of BSA and PEI were 40–60 μg mg⁻¹ and 12–15 μg mg⁻¹ in the BSA/PLGA NPs and PEI/PLGA NPs, respectively. About 13–18 μg mg⁻¹ BSA was exposed onto the surface of the BSA/PLGA NPs. No degradation of the PLGA NPs was detected after being incubated in artificial lysosomal fluid or with macrophages in a culture medium for 7 days. The innate immune activation behavior of the BSA/PLGA and PEI/PLGA NPs was evaluated by co-incubation with RAW264.7 cells in vitro. PLGA NPs fabricated with different molecular weights of PLGA showed no difference in stimulating RAW264.7 cells. The PEI/PLGA NPs did not show significant immune activation in terms of secretion of inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) too. By contrast, the BSA/PLGA NPs induced a significantly higher expression of TNF-α, likely due to the heterogeneous albumin and existence of endotoxin, and the synergistic role of larger uptake of the BSA/PLGA NPs by macrophages.

中文翻译：

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PLGA纳米颗粒表面涂层对巨噬细胞免疫激活的影响†

聚（丙交酯-共-乙交酯）（PLGA）是用于装载和输送有效载荷（尤其是纳米颗粒（NP）形式）的最具吸引力的生物可降解聚合物之一。在这项工作中，使用牛血清白蛋白（BSA）和聚乙烯亚胺（PEI）作为分散剂制备了3种不同分子量的PLGA NP。元素分析表明，BSA / PLGA NPs和PEI / PLGA NPs中BSA和PEI的负载量分别为40–60μgmg ^-1和12–15μgmg ^-1。约13–18μg毫克^-1BSA暴露在BSA / PLGA NP的表面上。在人工溶酶体液中或巨噬细胞在培养基中孵育7天后，未检测到PLGA NP的降解。的BSA / PLGA与PEI / PLGA NP的先天免疫激活行为通过共温育评价了RAW264.7细胞在体外。用不同分子量的PLGA制成的PLGA NP在刺激RAW264.7细胞方面没有差异。PEI / PLGA NPs在炎症性细胞因子如肿瘤坏死因子-α（TNF-α），白介素-6（IL-6）和白介素-1β（IL-1β）的分泌方面也没有显示出明显的免疫激活作用。 。相比之下，BSA / PLGA NPs诱导的TNF-α明显更高的表达，这可能是由于白蛋白异质和内毒素的存在，以及巨噬细胞对BSA / PLGA NPs的更大吸收的协同作用。