The study presents observations on anticancer therapeutic efficacy of magnetic fluid hyperthermia and a combination of hyperthermia and chemotherapy (i.e., integrative treatment) using an in vitro perfused and non-perfused 3D breast tumor model. The 3D in vitro breast tumor models were simulated using Comsol multiphysics, fabricated using specially designed chips, and treated with doxorubicin-loaded chitosan-coated La_0.7Sr_0.3MnO₃ (DC-LSMO) nanoparticles for hyperthermia and combination therapy in both perfused and non-perfused conditions. Computation confirmed uniform heat distribution throughout the scaffold for both the models. The findings indicate that both hyperthermia and combination treatment could trigger apoptotic cell death in the perfused and non-perfused models in varying degrees. Specifically, the perfused tumors were more resistant to therapy than the non-perfused ones. The efficacy of anticancer treatment decreased with increasing physiological complexity of the tumor model. The combination (hyperthermia and chemotherapy) treatment showed enhanced efficacy over hyperthermia alone. This is a pilot study to investigate the effects of magnetic fluid hyperthermia–chemotherapy treatment using perfused and non-perfused 3D in vitro models of tumor. The feasibility of using 3D cell culture models for contributing to our understanding of cancer and its treatment was also determined as a part of this work.

中文翻译：

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使用体外灌注使能的3D乳腺癌模型对综合抗癌治疗的评估

该研究使用体外灌注和非灌注3D乳腺肿瘤模型，对磁流体热疗以及热疗和化学疗法（即综合治疗）的组合的抗癌治疗效果进行了观察。使用Comsol多物理场模拟3D体外乳腺肿瘤模型，使用专门设计的芯片制造，并用阿霉素负载的壳聚糖涂层La _0.7 Sr _0.3 MnO _3处理（DC-LSMO）纳米粒子，用于灌注和非灌注条件下的热疗和联合治疗。计算证实了两个模型在整个支架上的热量分布均匀。研究结果表明，热疗和联合治疗均可在不同程度的灌注和非灌注模型中触发凋亡细胞死亡。具体而言，灌注肿瘤比未灌注肿瘤对治疗的抵抗力更高。随着肿瘤模型的生理复杂性的增加，抗癌治疗的效力降低。与单独的高温疗法相比，联合疗法（高温疗法和化学疗法）显示出更高的疗效。这是一项前瞻性研究，目的是研究使用灌注的和非灌注的3D体外肿瘤模型对磁流体高温化学疗法的治疗效果。