Biological samples are notoriously difficult to use within portable miniaturised devices, often due to complex sample matrices or variation between cellular samples. Herein we describe a device that utilises a correction based upon the total soluble cellular protein and absorbance measurements for the accurate quantification of cell number from equine buccal swabs. Hemocytometer cell counts were initially conducted to determine average mammalian and bacterial contributions (97.66% and 2.34% respectively). Secondly, cells were lysed and centrifuged before the supernatant was utilised within a Bradford's ratio metric absorbance assay using a microplate reader. A correlation coefficient of r = 0.992 was determined, indicating this method was successful in utilising total soluble cellular protein for the quantification of cell number in unknown biological samples of this type. Upon the identification of a strong linear correlation coefficient, a portable microfluidic device was developed (r = 0.983) as a highly specific platform for real time buccal cell number quantification with the ability to be used for sample correction for buccal swab samples as low as 7.28 μg mL⁻¹ in economic downstream biological diagnostic devices.

中文翻译：

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用于快速定量马颊拭子样本中细胞数量的简单设备†

众所周知，通常由于复杂的样品基质或细胞样品之间的差异，生物样品很难在便携式小型设备中使用。在这里，我们描述了一种装置，该装置利用基于总可溶性细胞蛋白和吸光度测量值的校正来准确定量马颊拭子的细胞数。最初进行血细胞计数器细胞计数以确定哺乳动物和细菌的平均贡献（分别为97.66％和2.34％）。其次，将细胞裂解并离心，然后使用酶标仪在Bradford比值吸光度测定法中使用上清液。r的相关系数= 0.992，表明该方法成功地利用总可溶性细胞蛋白定量了这种类型的未知生物学样品中的细胞数。在确定了很强的线性相关系数后，便开发了一种便携式微流控设备（r = 0.983），作为实时颊细胞数量定量的高度特异性平台，可用于对口腔拭子样品进行低至7.28的样品校正。经济型下游生物诊断设备中的μgmL ^-1。