In the present study, a new sandwich-like nanocomposite as a multifunctional smart nanocarrier for curcumin (Cur) targeted delivery and cell imaging was prepared by immobilization of gold nanoparticles on folic acid-modified dendritic mesoporous silica-coated reduced graphene oxide nanosheets (AuNPs@GFMS). The physical and chemical properties of the nanocomposite were investigated by atomic force microscopy (AFM), transmission electron microscopy (TEM), X-ray diffraction (XRD), UV-Vis, field-emission scanning electron microscopy (FE-SEM), Fourier transformation infrared (FT-IR), and Brunauer–Emmett–Teller (BET) surface area analysis. The nanocarrier exhibits a number of interesting properties, including good biocompatibility, biodegradability, and suitable surface area, which results in high drug loading capacity. In addition, this new drug delivery system showed sustained-release and pH-responsive properties. The in vitro cytotoxicity test of the free curcumin, free nanocarrier (AuNPs@GFMS), curcumin-loaded folate-conjugated nanocarriers (Cur-AuNPs@GFMS), and curcumin-loaded nanocarriers without folate-conjugation (Cur-AuNPs@GAMS) against two human cancer cell lines, including MCF-7 (human breast carcinoma cell lines) and A549 (human lung carcinoma cell lines) demonstrated that the therapeutic efficacy of Cur-AuNPs@GFMS is significantly greater than those of other compounds because the cancerous cells can uptake the folate-conjugated drug nanocarrier via a receptor-mediated mechanism. Fluorescence microscopic images and different staining techniques were also used to visualize the cellular uptake, anticancer activity, specific targeting ability, and photothermal potency of Cur-AuNPs@GFMS toward the MCF-7 cancer cells. The obtained results proved that the proposed system, Cur-AuNPs@GFMS, can be used as a potent anticancer agent in targeted cancer therapies for breast cancer.

中文翻译：

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将金纳米颗粒固定在叶酸偶联的树枝状介孔介孔二氧化硅涂层的还原氧化石墨烯纳米片上：用于姜黄素pH控制和靶向递送的新型纳米平台

在本研究中，通过将金纳米颗粒固定在叶酸修饰的树枝状介孔二氧化硅包覆的还原氧化石墨烯纳米片上（AuNPs @），制备了一种新型三明治状纳米复合材料，作为用于姜黄素（Cur）靶向递送和细胞成像的多功能智能纳米载体。 GFMS）。通过原子力显微镜（AFM），透射电子显微镜（TEM），X射线衍射（XRD），UV-Vis，场发射扫描电子显微镜（FE-SEM），傅里叶（Fourier）研究了纳米复合材料的物理和化学性质红外变换（FT-IR）和布鲁诺尔·埃默特·泰勒（BET）表面积分析。纳米载体表现出许多令人感兴趣的性质，包括良好的生物相容性，生物降解性和合适的表面积，这导致高的药物载量。此外，这种新的药物递送系统具有持续释放和pH响应特性。这游离姜黄素，游离纳米载体（AuNPs @ GFMS），负载姜黄素的叶酸偶联纳米载体（Cur-AuNPs @ GFMS）和没有叶酸结合的姜黄素负载纳米载体（Cur-AuNPs @ GAMS）的体外细胞毒性测试人类癌细胞系，包括MCF-7（人类乳腺癌细胞系）和A549（人类肺癌细胞系）证明，Cur-AuNPs @ GFMS的治疗效果明显优于其他化合物，因为癌细胞可以摄取叶酸偶联药物纳米载体通过受体介导的机制。还使用荧光显微镜图像和不同的染色技术来可视化Cur-AuNPs @ GFMS对MCF-7癌细胞的细胞摄取，抗癌活性，特异性靶向能力和光热潜能。获得的结果证明，所提出的系统Cur-AuNPs @ GFMS可作为有效的抗癌剂用于乳腺癌的靶向癌症治疗中。