A simple, isocratic HPLC method based on HILIC-WAX separation, has been developed for analyzing sulfated disaccharides of glycosaminoglycans (GAGs). To our best knowledge, this is the first successful attempt using this special phase in nano-HPLC–MS analysis. Mass spectrometry was based on negative ionization, improving both sensitivity and specificity. Detection limit for most sulfated disaccharides were approximately 1 fmol; quantitation limits 10 fmol. The method was applied for glycosaminoglycan profiling of tissue samples, using surface digestion protocols. This novel combination provides sufficient sensitivity for GAG disaccharide analysis, which was first performed using prostate cancer tissue microarrays. Preliminary results show that GAG analysis may be useful for identifying cancer related changes in small amounts of tissue samples (ca. 10 μg).

已经开发了一种基于HILIC-WAX分离的简单等度HPLC方法，用于分析糖胺聚糖（GAG）的硫酸化二糖。据我们所知，这是在纳米HPLC-MS分析中使用这种特殊相的首次成功尝试。质谱基于负离子化，可提高灵敏度和特异性。大多数硫酸化二糖的检出限约为1 fmol。定量限为10 fmol。该方法适用于使用表面消化方案的组织样品的糖胺聚糖谱分析。这种新颖的组合为GAG二糖分析提供了足够的灵敏度，该分析首先使用前列腺癌组织微阵列进行。初步结果表明，GAG分析可能有助于识别少量组织样本中与癌症相关的变化（ca.