Hemolysis is the rupture of red blood cells and constitutes the most common reason for unsuitable blood samples in the clinic. To detect hemolysis, one has to separate the hemoglobin in blood plasma from that in red blood cells. However, current methods entail centrifugation for cell-plasma separation, which is complex, time-consuming, and not easy to integrate into point-of-care (PoC) systems. Here, we demonstrate an optofluidic sensor composed of nanofilters on an optical waveguide, which enables evanescent-wave absorption measurement of hemoglobin in plasma with the capability of real-time inline detection on whole blood without extra sample preparation like centrifugation. Long-term testing with inline integration in a modified, commercial blood gas analyzer shows high reliability and repeatability of the measurements even with the presence of interference from bilirubin. We envision that the present work has large potential in improving diagnosis quality by enabling PoC hemolysis detection in blood gas analyzers and can also lend unique sensing capabilities to other applications dealing with complex turbid media.

中文翻译：

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用于全血在线溶血检测的光电传感器

溶血是红细胞的破裂，是临床上不适当的血液样本的最常见原因。为了检测溶血，必须将血浆中的血红蛋白与红细胞中的血红蛋白分开。然而，当前的方法需要离心进行细胞-血浆分离，这是复杂的，耗时的，并且不容易整合到即时护理（PoC）系统中。在这里，我们演示了一种由光波导上的纳米滤光片组成的光流体传感器，该传感器能够在血浆中进行血红蛋白的e逝波吸收测量，并且能够对全血进行实时在线检测，而无需进行额外的样品制备（例如离心分离）。在修改后的内联集成中进行长期测试，商用血气分析仪即使在存在胆红素干扰的情况下也显示出高可靠性和可重复性的测量结果。我们认为，通过在血气分析仪中启用PoC溶血检测，当前的工作在提高诊断质量方面具有巨大潜力，并且还可以为处理复杂浑浊介质的其他应用提供独特的传感功能。