Viral pathogenesis results from changes in host cells due to virus usurpation of the host cell and the innate cellular responses to thwart infection. We measured global changes in protein expression and localization in HIV-1 infected T-cells using subcellular fractionation and the Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH-MS) proteomic platform. Eight biological replicates were performed in two independent experimental series. In silico merging of both experiments identified 287 proteins with altered expression (p < .05) between control and infected cells- 172 in the cytoplasm, 84 in the membrane, and 31 in nuclei. 170 of the proteins are components of the NIH HIV interaction database. Multiple Reaction Monitoring and traditional immunoblotting validated the altered expression of several factors during infection. Numerous factors were found to affect HIV infection in gain- and loss-of-expression infection assays, including the intermediate filament vimentin which was found to be required for efficient infection.

病毒发病机理是由于宿主细胞被病毒侵占以及对挫败性感染的先天细胞应答所导致的宿主细胞变化所致。我们使用亚细胞分级分离和所有理论质谱（SWATH-MS）蛋白质组学平台的顺序窗口采集，测量了HIV-1感染的T细胞中蛋白质表达和定位的总体变化。在两个独立的实验系列中进行了八次生物学重复。在计算机上两项实验的合并确定了在对照细胞和受感染细胞之间287种蛋白的表达发生了改变（p <.05）-在细胞质中为172，在膜中为84，在细胞核中为31。其中170种蛋白质是NIH HIV相互作用数据库的组成部分。多重反应监测和传统的免疫印迹验证了感染过程中几种因子表达的改变。在表达增减感染试验中发现了许多因素会影响HIV感染，包括发现中间丝纤丝波形蛋白是有效感染所必需的。