Abnormal expression of sialylated Thomsen–Friedenreich antigen (Neu5Acα2-3Galβ1-3GalNAcα-O-Ser/Thr, sialyl-T) has a strong relationship with various types of human cancers and many other diseases. However, the size and structural complexity, and relatively lower abundance of sialyl-T have posed a significant challenge to its detection. Therefore, details about the role of sialyl-T in a variety of physiological and pathological processes are still poorly understood. Here, a one-step chemoenzymatic labeling strategy to probe sialyl-T is described. This approach enables the sensitive, selective, and rapid detection of sialyl-T, and global profiling and identification of unknown sialyl-T-attached glycoproteins, which are potential therapeutic targets or biomarkers. The use of one-step labeling strategy not only has a higher sensitivity than a typical two-step reporter strategy but also avoids undergoing an additional chemical reaction step to introduce a reporter group after the labeling reaction, making it particularly useful for detecting low-abundance glycan epitopes on living cells.

中文翻译：

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一步一步化学酶标记策略，用于探测唾液酸化的Thomsen–Friedenreich抗原。

唾液酸化的Thomsen–Friedenreich抗原（Neu5Acα2-3Galβ1-3GalNAcα-O-Ser/ Thr，唾液酸-T）的异常表达与各种类型的人类癌症和许多其他疾病有着密切的关系。然而，唾液酸基-T的尺寸和结构复杂性以及相对较低的丰度对其检测提出了重大挑战。因此，关于唾液酸-T在各种生理和病理过程中的作用的细节仍知之甚少。在这里，描述了一步化学探针标记策略来探测唾液酸-T。这种方法使得能够对唾液酸-T进行灵敏，选择性和快速的检测，并能够对潜在唾液酸-T糖蛋白（可能是潜在的治疗靶标或生物标记物）进行整体分析和鉴定。