当前位置: X-MOL 学术Anal. Methods › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Chemometrics-assisted liquid chromatography-full scan mass spectrometry for simultaneous determination of multi-class estrogens in infant milk powder†
Analytical Methods ( IF 3.1 ) Pub Date : 2018-02-23 00:00:00 , DOI: 10.1039/c7ay02733h
Xiao-Dong Sun 1, 2, 3, 4, 5 , Hai-Long Wu 1, 2, 3, 4, 5 , Zhi Liu 1, 2, 3, 4, 5 , Li-Xia Xie 1, 2, 3, 4, 5 , Yong Hu 1, 2, 3, 4, 5 , Huan Fang 1, 2, 3, 4, 5 , Tong Wang 1, 2, 3, 4, 5 , Rong Xiao 1, 2, 3, 4, 5 , Yu-Jie Ding 1, 2, 3, 4, 5 , Ru-Qin Yu 1, 2, 3, 4, 5
Affiliation  

In the present study, a smart analytical strategy that combines liquid chromatography-full scan mass spectrometry with the second-order calibration method based on the alternating trilinear decomposition (ATLD) algorithm was developed for the simultaneous determination of seven estrogens in infant milk powders. The seven estrogens were rapidly eluted out within 7.0 min under a simple gradient condition and then, they were detected by mass spectrometry operated in the full scan mode. With the aid of the prominent “second-order advantage” of the algorithm, specific qualitative and quantitative information about the target analytes could be extracted from the complex system even in the presence of considerable peak overlaps, baseline drifts and unknown interferences. The proposed strategy avoided time-consuming and laborious sample pretreatment procedures, resulting in minimal loss of analytes, which improved the analytical accuracy. Average recoveries of the seven estrogens in two spiked infant milk powder samples were in the range of 91.2–104.2% with the relative standard deviations (RSDs) lower than 5.0% (with the exception for 17α-estradiol), and the limits of detection (LOD) ranged from 0.07 to 2.49 ng mL−1. Besides, to further confirm the feasibility and reliability of the proposed method, the same batch of samples was analyzed using the LC-MS/MS method, and the statistical tests showed that no significant difference existed between the two methods, which fully indicated that the proposed strategy could provide satisfactory prediction results in real infant milk powder samples as well as other actual chemical systems.

中文翻译:

化学计量学辅助液相色谱-全扫描质谱法同时测定婴儿奶粉中的多种雌激素

在本研究中,开发了一种智能分析策略,该方法结合了液相色谱-全扫描质谱法和基于交替三线性分解(ATLD)算法的二阶校准方法,可同时测定婴儿奶粉中的七个雌激素。在简单的梯度条件下,在7.0分钟内将7种雌激素迅速洗脱出来,然后通过在全扫描模式下运行的质谱进行检测。借助该算法突出的“二阶优势”,即使存在大量的峰重叠,基线漂移和未知干扰,也可以从复杂系统中提取有关目标分析物的特定定性和定量信息。所提出的策略避免了费时费力的样品预处理程序,从而使分析物损失降至最低,从而提高了分析准确性。在两个加标的婴儿奶粉样品中,七个雌激素的平均回收率在91.2–104.2%的范围内,相对标准偏差(RSD)低于5.0%(17α-雌二醇除外),并且检出限( LOD)范围从0.07至2.49 ng mL-1。此外,为进一步证实该方法的可行性和可靠性,使用LC-MS / MS方法对同一批次的样品进行了分析,统计测试表明这两种方法之间没有显着差异,这充分表明了该方法的可行性。所提出的策略可以在实际的婴儿奶粉样品以及其他实际的化学系统中提供令人满意的预测结果。
更新日期:2018-02-23
down
wechat
bug