Abstract In this work, novel active edible films having antiviral activity were developed through the solvent casting method. Emulsified edible films were prepared by adding lipids into alginate films in order to improve their water vapour permeability. In addition, two natural extracts rich in phenolic compounds, specifically a green tea extract (GTE) and a grape seed extract (GSE) were used as active agents. Interestingly, incorporating GTE or GSE within the biopolymer matrix, the films exhibited significant antiviral activity against murine norovirus (MNV) and hepatitis A virus (HAV) since ∼2 log reduction were recorded for the 0.75 g extract/g alginate in the film, being GTE more efficient than GSE. Furthermore, the incorporation of phenolic compounds imparted antioxidant capacity to the active films, showing 14.79 ± 0.25 and 3.50 ± 0.25 mM Trolox⋅ g extract in the film−1 for 0.75 GTE and 0.75 GSE films, respectively. It was also found that water barrier efficiency improved upon addition of the highest amount of GTE or GSE (1:0.75 alginate:extract ratio) which was ascribed to molecular interactions between the films' components, as demonstrated by infrared spectroscopy assays. These results reveal the potential of these active films, mainly the ones containing GTE, to improve food quality and safety.

中文翻译：

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含有酚类提取物的活性藻酸盐可食用薄膜的抗病毒和抗氧化特性

摘要 在这项工作中，通过溶剂浇铸方法开发了具有抗病毒活性的新型活性可食用薄膜。通过在海藻酸盐薄膜中加入脂质以提高其水蒸气渗透性，制备乳化可食用薄膜。此外，两种富含酚类化合物的天然提取物，特别是绿茶提取物 (GTE) 和葡萄籽提取物 (GSE) 被用作活性剂。有趣的是，在生物聚合物基质中加入 GTE 或 GS​​E，这些薄膜对鼠诺如病毒 (MNV) 和甲型肝炎病毒 (HAV) 表现出显着的抗病毒活性，因为薄膜中 0.75 g 提取物/g 藻酸盐的记录减少了约 2 log，是GTE 比 GSE 更有效率。此外，酚类化合物的加入赋予活性薄膜抗氧化能力，分别为 14.79 ± 0.25 和 3.50 ± 0。对于 0.75 GTE 和 0.75 GSE 薄膜，薄膜-1 中的 25 mM Trolox·g 提取物分别。还发现添加最高量的 GTE 或 GS​​E（1:0.75 海藻酸盐：提取物比率）后，水阻隔效率提高，这归因于薄膜组分之间的分子相互作用，如红外光谱分析所证明的。这些结果揭示了这些活性薄膜（主要是含有 GTE 的活性薄膜）在改善食品质量和安全方面的潜力。