We herein systematically investigate the voltage-driven counting of phospholipid vesicles with single conical nanopipettes by resistive-pulse principle in liquid phase. With increasing the positive bias voltage, the translocation-event frequency increases and the dwell time decreases. We analyze the effect of applied voltage on nanoparticle dynamics based on the calculation of transfer frequency and translocation velocity of the vesicle at the nanopipette orifice and find that the applied voltage shows an obvious influence on analytical performance and can serve as a useful parameter to optimizing the analytical performance of vesicles. This study demonstrates that the nanopipette could be used as an easily operated platform for vesicle counting.

我们在本文中通过电阻脉冲原理在液相中系统地研究了具有单个锥形纳米移液器的磷脂囊泡的电压驱动计数。随着正偏置电压的增加，易位事件频率增加，并且停留时间减少。我们基于纳米吸管孔处囊泡的转移频率和转运速度的计算分析了施加电压对纳米粒子动力学的影响，发现施加电压对分析性能具有明显影响，可以作为优化分析效率的有用参数。囊泡的分析性能。这项研究表明，纳米吸管可以用作易于操作的囊泡计数平台。