A highly efficient method for aptamer screening with real-time monitoring of the SELEX process was described by silver decahedra nanoparticles (Ag₁₀-NPs) enhanced surface plasmon resonance imaging (SPRI). A microarray chip was developed by immobilization of target protein (Lactoferrin (Lac)) and control proteins (α-lactalbumin (α), β-lactoglobulin (β), casein, and bovine serum albumin (BSA)) on the biochip surface. Ag₁₀-NPs were conjugated with an ssDNA library (lib) (Ag₁₀-NPs-library) that consisted of a central 40 nt randomized sequence and a 20 nt fixed primer sequence. Introduction of the Ag₁₀-NPs-library to the SPRI flow channels drastically increased the sensitivity of SPRI signal for real-time monitoring of SELEX. The work allows rapid screening of potential targets, and yields nine aptamers with high affinity (nanomolar range) for Lac after only six-rounds of selection. The aptamer Lac 13-26 was then further tested by SPRI, and the results demonstrated that the aptamer had the capacity to be ultra-sensitive for specific detection of Lac. The novel SPRI-SELEX method demonstrated here showed many advantages of real-time evaluation, high throughput, and high efficiency.

银十面体纳米粒子（Ag ₁₀ -NPs）增强的表面等离子体共振成像（SPRI）描述了一种通过实时监测SELEX过程进行适体筛选的高效方法。通过将靶蛋白（乳铁蛋白（Lac）和固定蛋白（α-乳白蛋白（α），β-乳球蛋白（β），酪蛋白和牛血清白蛋白（BSA））固定在生物芯片表面来开发微阵列芯片。Ag ₁₀ -NP与一个ssDNA文库（lib）（Ag ₁₀ -NPs-library）偶联，该文库由一个中央40 nt随机序列和一个20 nt固定引物序列组成。Ag _10的介绍SNP流动通道的-NPs-library大大提高了SPRI信号的实时监测SELEX的灵敏度。这项工作可以快速筛选潜在的靶标，仅经过六轮选择，即可产生九个对Lac具有高亲和力（纳摩尔范围）的适体。然后，通过SPRI对适体Lac 13-26进行了进一步测试，结果表明，适体具有对Lac进行特异性检测的超灵敏能力。这里展示的新颖的SPRI-SELEX方法显示了实时评估，高吞吐量和高效率的许多优点。