Lysosomal polarity affects the interaction activities between enzymes and substrates at the cellular level. Abnormal lysosomal polarity closely linked with disorders and diseases is worthy of attention. The first fluorescence probe, which can image polarity ratiometrically and detect lysosomal polarity quantitatively, is reported herein. The probe termed NOH can emit dual-peaks both in solvents (λ_em = 474, 552 nm) and in micro-environment. NOH exhibits the Boltzmann function response of the fluorescence intensity ratio to the polarity in a wide range and localizes at lysosomes specifically (R_r = 0.97). In the method of ratiometric fluorescence imaging with NOH, the variation of lysosomal polarity (Δf) can be directly discerned by the color changes. In virtue of ratiometric fluorescence imaging and the Boltzmann function relationship between the fluorescence intensity ratio and the polarity, lysosomal polarity in MCF-7 cells was calculated to be 0.224 and the polarity in the condition of lysosomal storage disorders (or cell death) could also be obtained. This probe will be a promising tool for studying lysosome-related physiological or pathological processes.

溶酶体极性在细胞水平上影响酶和底物之间的相互作用。与疾病和疾病密切相关的溶酶体极性异常值得关注。本文报道了可以按比例成像极性并定量检测溶酶体极性的第一荧光探针。称为NOH的探针可以在溶剂（_λem  = 474，552 nm）和微环境中发射双峰。NOH在宽范围内显示出荧光强度比对极性的玻尔兹曼函数响应，并且特异性地位于溶酶体上（R _r  = 0.97）。用NOH进行比例荧光成像的方法，溶酶体极性（Δf）的变化可以通过颜色变化直接辨别。借助比例荧光成像和荧光强度比与极性之间的玻尔兹曼函数关系，计算出MCF-7细胞中的溶酶体极性为0.224，在溶酶体贮积障碍（或细胞死亡）的情况下，极性也可能为0.224。获得。该探针将成为研究溶酶体相关的生理或病理过程的有前途的工具。