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Extreme thermal stability of Lactococcus lactis bacteriophages: Evaluation of phage inactivation in a pilot-plant pasteurizer
LWT - Food Science and Technology ( IF 6 ) Pub Date : 2018-02-23 , DOI: 10.1016/j.lwt.2018.02.056
Natalia Wagner , Sönke Matzen , Hans-Georg Walte , Horst Neve , Charles M.A.P. Franz , Knut J. Heller , Philipp Hammer

Thermal inactivation of highly heat-stable Lactococcus lactis phages P680 and P1532 of the 936 group and P635 of the c2 group was investigated in a pilot-plant pasteurizer after suspension of the phages in raw milk (107 pfu/ml). The heat-sensitive reference phage P008 was tested as a control. The titers of the phages P008 and P635 decreased ≥99.99% after heating for 32 s at 75 °C and 80 °C, respectively. To achieve a similar inactivation of ≥99.99%, phages P680 and P1532 had to be treated for 39 s at temperatures of 95 °C and 97.5 °C, respectively. D- and z-values for thermal inactivation were calculated for each of the phages. When comparing thermal inactivation data of phages in the pilot-plant pasteurizer obtained in this study with literature data on phage heat inactivation in stainless-steel test tubes in a water bath, the pilot-plant pasteurizer was notably more efficient.



中文翻译:

乳酸乳球菌噬菌体的极高热稳定性:中试巴氏灭菌器中噬菌体灭活的评估

在中试巴氏灭菌器中,将噬菌体悬浮在生牛奶中(10 7  pfu / ml)后,研究了936组的高度热稳定的乳酸乳球菌噬菌体P680和P1532以及c2组的P635的热失活。测试热敏参考噬菌体P008作为对照。在75°C和80°C加热32 s后,噬菌体P008和P635的滴度分别降低≥99.99%。为实现≥99.99%的相似灭活,噬菌体P680和P1532必须分别在95°C和97.5°C的温度下处理39 s。D-z计算每个噬菌体的热失活值。将本研究中获得的中试巴氏灭菌器中噬菌体的热灭活数据与水浴中不锈钢试管中噬菌体热失活的文献数据进行比较时,中试巴氏灭菌器的效率明显提高。

更新日期:2018-02-23
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