Bisphenol A (BPA), a contaminant of emerging concern, can affect plant growth and development at high concentrations. Reactive oxygen species (ROS) production is a general primary response in plants to stress. Here, the aim is to investigate whether ROS in plants play protective roles for stress induced by BPA exposure at environmental concentrations. In this study, soybean roots (seedling, flowering and podding stages) were exposed to 1.5 and 3.0 mg L⁻¹ BPA, and ROS response was measured. The relationship between ROS levels and residual BPA content in soybean roots was evaluated. The results showed that exposure (9 h) to 1.5 mg L⁻¹ BPA elicited changes in ROS production. ROS then gradually accumulated in soybean roots (seedling stage). Exposure to 3.0 mg L⁻¹ BPA elicited a stronger and earlier ROS responses at the flowering and podding stage, but did not lead to membrane lipid peroxidation. Residual BPA content in soybean roots reached peak concentrations after 9 h of exposure, and then gradually decreased at the flowering and podding stage. These results indicate that ROS in soybean roots might be involved in the oxidative metabolism of BPA, which could prevent BPA from damaging exposed plants. In conclusion, the observed ROS metabolic effects may be self-protection responses of plants to stress induced by BPA exposure.

双酚A（BPA）是一种新出现的污染物，在高浓度下会影响植物的生长发育。活性氧（ROS）的产生是植物对逆境的一般主要反应。在这里，目的是研究植物中的ROS是否在环境浓度下对BPA暴露诱导的逆境起到保护作用。在这项研究中，将大豆根部（幼苗，开花和荚果期）暴露于1.5和3.0 mg L ^-1 BPA中，并测量了ROS响应。评估了大豆根中ROS水平与残留BPA含量之间的关系。结果表明，暴露于1.5 mg L ^-1 BPA （9 h）会引起ROS产生变化。ROS然后逐渐积累在大豆根中（苗期）。暴露于3.0 mg L ^-1BPA在花期和荚果期引起更强和更早的ROS反应，但没有导致膜脂质过氧化。暴露9 h后，大豆根中残留的BPA含量达到峰值，然后在开花和结荚期逐渐降低。这些结果表明大豆根中的ROS可能参与了BPA的氧化代谢，这可以防止BPA损害暴露的植物。总之，观察到的ROS代谢效应可能是植物对BPA暴露诱导的胁迫的自我保护反应。