Salmonella is a major foodborne pathogen throughout the world and able to resist extreme acid stress such as stomach gastric juice after mild acid adaptation, which makes it a potential hazard in food industry. We conducted a comparative transcriptomic analysis by high-throughout RNA-seq to elucidate the Acid Tolerance Response (ATR) in Salmonella Enteritidis. Among these 554 differentially expressed genes (DEGs) identified in this study, the expression of 280 DEGs were up-regulated and 274 DEGs were down-regulated. First, S. Enteritidis tried to survive the adverse environment by reducing the energy-consuming metabolisms and maintaining essential processes as an energy conservation strategy. Second, ATR S. Enteritidis underwent damages in DNA, an oxidization damage result in an iron-lacking circumstance and so on. Thus DEGs related to Fe/S cluster biogenesis, stress response regulating proteins and transport proteins were responsive to acid stress to repair damage caused by acid and ROS. Last, to balance extra protons, by increasing the intracellular NAD (+)/NADH ratio, S. Enteritidis could also decline the protein acetylation level by promoting the consumption of acetyl coenzyme A via TCA, to prevent the intracellular pH from further decline under acid stress.

沙门氏菌是全世界主要的食源性病原体，经过适度的酸适应后能够抵抗极端的酸胁迫，例如胃液，这使其在食品工业中具有潜在的危害。我们通过高通量RNA-SEQ进行了比较分析转录以阐明在酸耐受反应（ATR）沙门氏菌肠炎。在这项研究中鉴定的这554个差异表达基因（DEG）中，有280个DEG的表达上调，而有274个DEG的表达下调。首先，S.肠炎试图通过降低耗能代谢和维持基本流程为节能减排战略生存的恶劣环境。二，ATR S。肠炎杆菌的DNA受到损伤，氧化损伤导致缺乏铁的情况，等等。因此，与Fe / S团簇生物发生，应激反应调节蛋白和转运蛋白有关的DEG对酸应激有响应，以修复由酸和ROS引起的损伤。最后，以平衡额外质子，通过提高细胞内NAD（+）/ NADH比率，S.肠炎也可通过促进乙酰辅酶A通过TCA的消耗下降的蛋白乙酰化水平，以防止在酸进一步下降的细胞内pH压力。