A new type of magnetic nanoparticles (MNPs), as the absorbents of bisphenol A (BPA), was prepared by functionalization of Fe₃O₄@SiO₂ with BPA-specific aptamer in this work. ssDNA aptamer was immobilized on the Fe₃O₄@SiO₂ surface through biotin-avidin interactions, playing a role of the specific probe for BPA. The resultant materials (Apt-MNPs) exhibited outstanding magnetic responsibility and can be separated efficiently by the magnetic field. Experimental results also showed that Apt-MNPs had large adsorption capacity and high competitive selectivity for the targeted compound BPA. Furthermore, Apt-MNPs were adopted as the specific absorbents to extract and enrich BPA from human serum and urine samples. Therefore, an efficient detection method of BPA was developed in combination with high-performance liquid chromatography (HPLC). The linearity of the method was over a range of 5–10,000 ng mL⁻¹ with a correlation coefficient of 0.99997, and the limit of detections (LODs) for serum and urine were 2.0 and 1.0 ng mL⁻¹, respectively. The recoveries of BPA in the spiked human serum and urine samples were 90.8 ± 7.3% (RSD) and 92.3 ± 1.5%, respectively. Our results demonstrated that Apt-MNPs were high-performance adsorbents for extracting and enriching BPA, resulting in fast and efficient detection of BPA in serum and urine samples.

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在这项工作中，通过用BPA特异性适体对Fe ₃ O ₄ @SiO ₂进行功能化，制备了一种新型的磁性纳米颗粒（MNPs），作为双酚A（BPA）的吸收剂。ssDNA适体被固定在Fe ₃ O ₄ @SiO _2上通过生物素-抗生物素蛋白的相互作用形成表面，从而发挥BPA特异性探针的作用。所得材料（Apt-MNP）表现出出色的磁性，可以被磁场有效分离。实验结果还表明，Apt-MNPs对目标化合物BPA具有较大的吸附能力和较高的竞争选择性。此外，采用Apt-MNPs作为特异性吸收剂，从人血清和尿液样品中提取和富集BPA。因此，结合高效液相色谱（HPLC），开发了一种有效的BPA检测方法。该方法的线性范围为5–10,000 ng mL ^-1，相关系数为0.99997，血清和尿液的检出限（LOD）为2.0和1.0 ng mL^-1。加标的人血清和尿液样品中BPA的回收率分别为90.8±7.3％（RSD）和92.3±1.5％。我们的结果表明，Apt-MNPs是用于提取和富集BPA的高性能吸附剂，可快速有效地检测血清和尿液样品中的BPA。

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