Analytical and Bioanalytical Chemistry ( IF 4.3 ) Pub Date : 2018-01-20 , DOI: 10.1007/s00216-018-0853-9 Nadezhda A. Byzova , Alexandr E. Urusov , Anatoly V. Zherdev , Boris B. Dzantiev
The format of an immunochromatographic multiassay is first proposed with native antisera and a universal conjugate of antispecies antibodies with gold nanoparticles. This format allows (1) the exclusion of purification and conjugation stages for specific antibodies and (2) significant reduction of the concentration of specific antibodies in the system. The independent use of specific antibodies and a conjugated marker provided a low detection limit and high signal intensity. The proposed format was implemented for the simultaneous detection of two herbicides. The instrumental limits for the detection of atrazine and chlorsulfuron were 0.1 and 0.7 ng/mL, respectively, and the analysis time was 20 min. The suitability of the test system for monitoring these herbicides in nontreated apple and blackcurrant juices is shown. The assay technique is simple, sensitive, and easily transferrable to any other antigen.
The proposed format of the immunochromatographic multiassay is based on the use of native antisera and a universal conjugate of antispecies antibodies with gold nanoparticles. In this way purification and conjugation stages for specific antibodies are excluded, and the concentrations of specific antibodies and the conjugated marker can be varied independently to obtain a low detection limit.
中文翻译：
基于未加工抗血清的多重高灵敏度免疫色谱分析
免疫层析多测定法的格式首先是用天然抗血清和抗物种抗体与金纳米颗粒的通用结合物提出的。这种格式允许（1）排除特异性抗体的纯化和偶联阶段，以及（2）显着降低系统中特异性抗体的浓度。特异性抗体和偶联标记物的独立使用提供了低检测限和高信号强度。提议的格式用于同时检测两种除草剂。阿特拉津和氯磺隆的检测限分别为0.1 ng / mL和0.7 ng / mL，分析时间为20分钟。显示了用于监测未经处理的苹果汁和黑加仑汁中这些除草剂的测试系统的适用性。
免疫层析多重测定的拟议格式基于使用天然抗血清以及抗物种抗体与金纳米颗粒的通用结合物。以此方式，排除了特异性抗体的纯化和缀合阶段，并且特异性抗体和缀合的标记物的浓度可以独立地变化以获得低检测限。
京公网安备 11010802027423号