Abstract In this paper, the preliminary results of a study aimed to discriminate between the different species of lichens, from which orchil dyes originate, are presented. A multi-analytical approach has been used to analyze several species of lichens, in particular Roccella tinctoria DC, Lasallia pustulata L. and Ochrolechia tartarea L., in an attempt to identify distinguishing markers within different orchil mixtures. In the first part of the research, samples of yarns dyed with the different species, prepared in laboratory after the proper raw lichens treatment, have been subjected to SERS on fiber analyses. Hence an innovative ammonia protocol has been applied to all the specimens. Firstly, in order to evaluate the difference among the different species, HPTLC has been applied to the extracts of dyed yarns and the dye baths. All the spots present on the TLC plates have been analyzed through SERS (HPTLC-SERS), in order to obtain a SERS database of each single compound, fixed on the yarns. Then, HPTLC-MS and HPLC-MS experiments have been carried out, in order to deep into the identification of compounds corresponding to each spot.

中文翻译：

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新的先进提取和分析方法用于区分地衣染色纱线中使用的不同地衣物种：初步结果

摘要 在本文中，介绍了一项旨在区分不同种类地衣的研究的初步结果，兰花染料来源于这些地衣。一种多分析方法已被用于分析几种地衣物种，特别是 Roccella tinctoria DC、Lasallia pustulata L. 和 Ochrolechia tartarea L.，以尝试识别不同兰花混合物中的区分标记。在研究的第一部分，用不同物种染色的纱线样品，在适当的原始地衣处理后在实验室制备，对纤维进行 SERS 分析。因此，创新的氨协议已应用于所有标本。首先，为了评估不同物种之间的差异，HPTLC 已应用于染色纱线的提取物和染浴。TLC 板上存在的所有斑点都已通过 SERS (HPTLC-SERS) 进行分析，以获得固定在纱线上的每种单一化合物的 SERS 数据库。然后，进行了HPTLC-MS和HPLC-MS实验，以深入识别每个点对应的化合物。