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Heat stress-induced reactive oxygen species participate in the regulation of HSP expression, hyphal branching and ganoderic acid biosynthesis in Ganoderma lucidum
Microbiological Research ( IF 6.7 ) Pub Date : 2018-02-19 , DOI: 10.1016/j.micres.2018.02.006
Rui Liu , Xue Zhang , Ang Ren , Deng-Ke Shi , Liang Shi , Jing Zhu , Han-Shou Yu , Ming-Wen Zhao

Heat stress (HS) is an important environmental factor that affects the growth and metabolism of edible fungi, but the molecular mechanism of the heat stress response (HSR) remains unclear. We previously reported that HS treatment increased the length between two hyphal branches and induced the accumulation of ganoderic acid biosynthesis and the gene expression of heat shock proteins (HSPs) in Ganoderma lucidum. In this study, we found that HS induced a significant increase in the cytosolic ROS concentration, and exogenously added ROS scavengers NAC, VC and NADPH oxidase (Nox) inhibitor DPI reduce the cytosolic ROS accumulation in G. lucidum. In addition, the phenomena of the increased gene expression and increased length between the two hyphal branches and the accumulation of GA biosynthesis induced by HS were mitigated. Furthermore, we investigated the effects of HS on Nox-silenced strains (NoxABi-10, NoxABi-11 and NoxRi-4, NoxRi-7) and found that the level of ROS concentration was lower than that in wild-type (WT) strains treated with HS. Additionally, Nox silenced strains reduced the HS-induced increase in HSP expression, the length between two hyphal branches and GA biosynthesis compared with the WT strain. These data indicate that HS-induced ROS participate in the regulation of HSP expression, hyphal branching and ganoderic acid biosynthesis in G. lucidum. In addition, these findings identified potential pathways linking ROS networks to HSR, physiological and metabolic processes in fungi and provide a valuable reference for studying the role of ROS in HSR, mycelium growth and secondary metabolites.



中文翻译:

热应力引起的活性氧物质参与HSP表达,菌丝分支和灵芝酸生物合成的调节灵芝

热应激(HS)是影响食用菌生长和代谢的重要环境因素,但热应激反应(HSR)的分子机制仍不清楚。我们以前报道,HS治疗增加2个菌丝分支之间的长度和诱导的灵芝酸生物合成的积累和热休克蛋白的基因表达(HSP的)灵芝。在这项研究中,我们发现HS诱导胞质ROS浓度显着增加,并外源添加ROS清除剂NAC,VC和NADPH氧化酶(Nox)抑制剂DPI降低了灵芝中胞质ROS的积累。另外,减轻了由HS诱导的两个菌丝分支之间的基因表达增加和长度增加以及GA生物合成的积累的现象。此外,我们研究了HS对Nox沉默菌株(NoxABi-10,NoxABi-11和NoxRi-4,NoxRi-7)的影响,发现ROS浓度水平低于野生型(WT)菌株用HS治疗。此外,与野生型相比,Nox沉默菌株减少了HS诱导的HSP表达增加,两个菌丝分支之间的长度以及GA生物合成。这些数据表明HS诱导的ROS参与了灵芝中HSP表达,菌丝分支和灵芝酸生物合成的调节。另外,这些发现确定了ROS网络与HSR,真菌的生理和代谢过程相关的潜在途径,并为研究ROS在HSR,菌丝体生长和次级代谢产物中的作用提供了有价值的参考。

更新日期:2018-02-19
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