During the last decade, β-blockers such as metoprolol (MTP) have been frequently detected in surface water, aquatic systems and municipal water at concentrations of ng/L to μg/L. Only a small number of studies exist on the toxic effects induced by this group of pharmaceuticals on aquatic organisms. Therefore, the present study aimed to evaluate the oxidative damage induced by MTP in the common carp Cyprinus carpio, using oxidative stress biomarkers. To this end, indicators of cellular oxidation such as hydroperoxide content (HPC), lipid peroxidation (LPX) and protein carbonyl content (PCC) were determined, as well as the activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). Also, concentrations of MTP and its metabolite O-desmethyl metoprolol were determined in water as well as carp gill, liver, kidney, brain and blood, along with the partial uptake pattern of these compounds. Results show that carp takes up MTP and its metabolite in the different organs evaluated, particularly liver and gill. The oxidative stress biomarkers, HPC, LPX, and PCC, as well as SOD and CAT activity all increased significantly at most exposure times in all organs evaluated. Results indicate that MTP and its metabolite induce oxidative stress on the teleost C. carpio and that the presence of these compounds may constitute a risk in water bodies for aquatic species.

在过去十年中，β受体阻滞剂，例如美托洛尔（MTP），经常在地表水，水生系统和市政用水中被检测出，浓度范围为ng / L至μg/ L。关于这组药物对水生生物引起的毒性作用的研究很少。因此，本研究的目的是评估在鲤鱼诱导MTP氧化损伤鲤，使用氧化应激生物标记。为此，确定了细胞氧化的指标，例如过氧化氢含量（HPC），脂质过氧化（LPX）和蛋白质羰基含量（PCC），以及抗氧化酶超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性。 。此外，还测定了水中以及鲤鱼ill，肝，肾，脑和血液中MTP及其代谢产物O-去甲基美托洛尔的浓度，以及这些化合物的部分吸收方式。结果表明，在评估的不同器官中，鲤鱼吸收了MTP及其代谢产物，尤其是肝脏和g。氧化应激生物标志物，HPC，LPX和PCC以及SOD和CAT活性在所有评估器官的大多数暴露时间均显着增加。鲤鱼（C. carpio）以及这些化合物的存在可能在水体中构成水生生物种的风险。