This study aimed to extract and separate total anthocyanins from Lycium ruthenicum Murr. by combining deep eutectic solvents (DES) with macroporous resin chromatography and to develop green analytical methods for the determination of anthocyanins. Choline chloride/1,2-propanediol (1 : 2, mol mol⁻¹) containing 10% water (v/v) was screened as the optimal extraction solvent, and extraction at 52 °C for 45 min using a liquid/solid ratio of 20 : 1 gave the maximum extraction yield of total anthocyanins (4.45 ± 0.07 mg g⁻¹). According to the static/dynamic adsorption and desorption tests, LS-32 macroporous resin has the ability to separate anthocyanins from DES with a recovery ratio above 95%. An off-line heart-cutting two-dimensional HPLC-DAD/ESI-MS method was developed for the qualitative and quantitative analysis of L. ruthenicum anthocyanins using environmentally friendly mobile phases. Ethanol and a tartaric acid solution (0.3 mol L⁻¹) were used as the mobile phases of the first-dimensional HPLC-DAD method, and 15 anthocyanin peaks were detected within 17 min. The second-dimensional HPLC-MS method was used to identify the main anthocyanin peaks in the first dimension. Method validation showed that the developed method was accurate, stable and reliable for the analysis of L. ruthenicum anthocyanins. The present study provides an excellent alternative green method for the preparation and determination of anthocyanins from plant material.

中文翻译：

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基于低共熔溶剂的萃取物与绿色二维HPLC-DAD-ESI-MS / MS联用，用于测定黑枸杞中的花色苷。水果†

这项研究旨在从枸杞中提取和分离总花青素。通过将深共熔溶剂（DES）与大孔树脂色谱法结合使用，并开发了用于测定花色苷的绿色分析方法。筛选含有10％水（v / v）的氯化胆碱/ 1,2-丙二醇（1：2，mol mol ^-1）作为最佳提取溶剂，并以液/固比在52°C下提取45分钟20：1的比例可获得总花青素的最大提取率（4.45±0.07 mg g ^-1）。根据静态/动态吸附和解吸测试，LS-32大孔树脂具有将花青素与DES分离的能力，回收率超过95％。开发了一种离线心脏切割二维HPLC-DAD / ESI-MS方法，用于使用环境友好的流动相定性和定量分析钌色花青素。使用乙醇和酒石酸溶液（0.3 mol L ^-1）作为一维HPLC-DAD方法的流动相，在17分钟内检测到15个花色苷峰。二维HPLC-MS方法用于鉴定第一维中的主要花色苷峰。方法验证表明，所开发的方法准确，稳定，可靠。花青素。本研究为从植物材料中制备和测定花色苷提供了一种极好的绿色替代方法。