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Topical delivery of L-theanine ameliorates TPA-induced acute skin inflammation via downregulating endothelial PECAM-1 and neutrophil infiltration and activation
Chemico-Biological Interactions ( IF 5.1 ) Pub Date : 2018-02-17
Wei-Jie Zeng, Zhi Tan, Xing-Fei Lai, Ya-Nan Xu, Chun-Lin Mai, Jun Zhang, Zhen-Jia Lin, Xian-Guo Liu, Shi-Li Sun, Li-Jun Zhou

l-theanine, the most abundant free amino acid in tea, has been documented to possess many different bioactive properties through oral or intragastrical delivery. However, little is known about the effect of topical delivery of l-theanine on acute inflammation. In the present study, by using 12-O-tetradecanoylphorbol-13-acetate (TPA, 2.5 μg/ear)-induced ear edema model in mice, we first found that single-dose local pretreatment of l-theanine 30 min before TPA time- and dose-dependently suppressed the increases in both skin thickness and weight. Subsequently l-theanine ameliorated TPA-induced erythema, vascular permeability increase, epidermal and dermal hyperplasia, neutrophil infiltration and activation via downregulating the expression of PECAM-1 (a platelet endothelial adhesion molecule-1) in blood vessels and the production of pro-inflammatory cytokines IL-1β, TNF-α, and mediator cyclooxygenase-2 (COX-2), which is mainly expressed in neutrophils. It highlighted the potential of l-theanine as a locally administrable therapeutic agent for acute cutaneous inflammation.



中文翻译:

L-茶氨酸的局部给药可通过下调内皮PECAM-1和中性粒细胞的浸润和活化来改善TPA诱导的急性皮肤炎症

l-茶氨酸是茶中最丰富的游离氨基酸,已被证明通过口服或胃内给药具有许多不同的生物活性。但是,关于局部递送1-茶氨酸对急性炎症的影响知之甚少。在本研究中,通过使用12-O-十四烷酰-13-乙酸酯(TPA,2.5微克/耳)在小鼠中诱导的耳水肿模型中,我们首次发现单次剂量的局部预处理前30分钟TPA时间-theanine -并且剂量依赖性地抑制了皮肤厚度和重量的增加。随后l茶碱通过下调血管中PECAM-1(血小板内皮粘附分子-1)的表达和促炎细胞因子IL的产生,改善了TPA诱导的红斑,血管通透性增加,表皮和真皮增生,中性粒细胞浸润和活化。 -1β,TNF-α和介质环氧合酶2(COX-2),主要在嗜中性粒细胞中表达。它强调了1-茶氨酸作为局部可用于急性皮肤炎症的治疗剂的潜力。

更新日期:2018-02-17
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