Affinity-based methods using immobilized proteins are important approaches for understanding the interactions between small molecules and biological targets. This review is intended to provide an overview of different affinity based separation methods that have been applied to the study of peroxisome proliferator activated receptors (PPARs). The screening of compound to increase screening rates for synthetic and natural ligands of PPAR are reported. Pros and cons of the approaches in ligand discovery initiatives are discussed.

中文翻译：

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用于研究生物相互作用的基于亲和的分离方法：药物发现中过氧化物酶体增殖物激活受体的案例

使用固定蛋白的基于亲和力的方法是了解小分子与生物靶标之间相互作用的重要方法。本综述旨在概述不同的基于亲和力的分离方法，这些方法已应用于过氧化物酶体增殖物激活受体 (PPAR) 的研究。报道了化合物的筛选以提高对合成和天然 PPAR 配体的筛选率。讨论了配体发现计划中方法的优缺点。