Here, we utilized the ultrasonic emulsification technique to generate hyaluronic acid microspheres incorporating a fluorescence-based glucose biosensor. We synthesized a novel lanthanide ion luminophore based on Eu³⁺. Eu sulfosuccinimidyl dextran (Eu-dextran) and Alexa Fluor 647 sulfosuccinimidyl-ConA (Alexa Fluor 647-ConA) were encapsulated in hyaluronic acid hydrogel to generate microspheres. Glucose sensing was carried out using a fluorescence resonance energy transfer (FRET)-based assay principle. A proportional fluorescence intensity increase was found within a 0.5–10-mM glucose concentration range. The glucose-sensing strategy showed an excellent tolerance for potential interferents. Meanwhile, the fluorescent signal of hyaluronic acid microspheres was very stable after testing for 72 h in glucose solution. Overall, hyaluronic acid microspheres encapsulating sensing biomolecules offer a stable and biocompatible biosensor for a variety of applications including cell culture systems, tissue engineering, detection of blood glucose, etc.

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在这里，我们利用超声乳化技术生成结合了基于荧光的葡萄糖生物传感器的透明质酸微球。我们基于Eu ³⁺合成了一种新型镧系离子发光体。将Eu磺基琥珀酰亚胺基葡聚糖（Eu-dextran）和Alexa Fluor 647磺基琥珀酰亚胺基-ConA（Alexa Fluor 647-ConA）封装在透明质酸水凝胶中以生成微球。使用基于荧光共振能量转移（FRET）的测定原理进行葡萄糖感测。在0.5-10mM的葡萄糖浓度范围内发现了成比例的荧光强度增加。葡萄糖传感策略显示出对潜在干扰物的极好的耐受性。同时，在葡萄糖溶液中测试72小时后，透明质酸微球的荧光信号非常稳定。总体而言，封装感测生物分子的透明质酸微球为各种应用提供了稳定且生物相容的生物传感器，包括细胞培养系统，组织工程，血糖检测等。

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