Deoxynivalenol (DON) is one of the most prevalent fusarium mycotoxins in feedstuff and food. DON causes detrimental effects on human and animal reproductive systems by inducing oxidative stress and apoptosis. However, melatonin is a multifunctional endogenous hormone that plays crucial roles in the development of animal germ cells and embryos as a robust deoxidizer. In this study, we explored the effects of melatonin on the DON exposure mouse oocytes. Our in vitro and in vivo results showed that DON adversely affected mouse oocyte maturation and early embryo cleavage, while melatonin administration ameliorated the toxic effects of DON. DON exposure disrupted the meiotic spindle formation and kinetochore‐microtubule attachment, which induced aneuploidy in oocytes. This might be through DON effects on the acetylated tubulin level. Moreover, we found that DON exposure caused the alteration of DNA and histone methylation level, which might affect early embryo cleavage. The toxic effects of DON on oocytes might be through its induction of oxidative stress‐mediated early apoptosis, while the treatment with melatonin significantly ameliorated these phenotypes in DON‐exposed mouse oocytes. Collectively, our results indicated the protection effects of melatonin against defects induced by DON during mouse oocyte meiotic maturation.

中文翻译：

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褪黑素可防止小鼠卵母细胞成熟过程中脱氧雪茄烯醇引起的缺陷

脱氧雪腐烯醇（DON）是饲料和食品中最普遍的镰刀菌霉菌毒素之一。DON通过诱导氧化应激和细胞凋亡对人类和动物生殖系统产生有害影响。但是，褪黑激素是一种多功能的内源激素，在动物生殖细胞和胚胎的发育中起着至关重要的脱氧剂的作用。在这项研究中，我们探讨了褪黑激素对DON暴露小鼠卵母细胞的影响。我们的体外和体内结果表明，DON不利地影响了小鼠卵母细胞的成熟和早期胚胎的裂解，而褪黑激素的给药改善了DON的毒性作用。DON暴露破坏了减数分裂纺锤体的形成和线粒体-微管的附着，从而诱导了卵母细胞的非整倍性。这可能是由于DON对乙酰化微管蛋白水平的影响。而且，我们发现DON暴露会引起DNA和组蛋白甲基化水平的改变，这可能会影响早期胚胎的切割。DON对卵母细胞的毒性作用可能是通过其诱导氧化应激介导的早期细胞凋亡，而褪黑激素的治疗​​显着改善了DON暴露的小鼠卵母细胞中的这些表型。总的来说，我们的结果表明褪黑激素对小鼠卵母细胞减数分裂成熟期间由DON引起的缺陷的保护作用。