Unlike reported bisindoles linked by single bond directly, alstoniasidines A (1) and B (2), from Alstonia scholaris featuring unprecedented skeleton with two indole moieties bridged by a sugar, represented a novel bisindole type having strictosamide-glucopyranose-picraline scaffold. Both compounds exhibited selective cytotoxicity against human glioma stem cells (GSCs) and induced caspase-3 dependent extrinsic apoptosis by increasing the expression of interleukin 1 (IL-1), tumor necrosis factor (TNF-α), and the cleaved caspase-3, while damaged the unlimited proliferation and self-renewal capacity of GSCs. This finding might provide new type of leads for the selective killing of human glioma stem cells.

与报道的通过单键直接连接的双吲哚不同，来自Alstonia Scholaris的alstoniasidines A（1）和B（2）具有前所未有的骨架，带有两个由糖桥接的吲哚部分，代表了一种新型双吲哚类型，其具有严格的酰胺-吡喃葡萄糖-吡啶骨架。两种化合物均通过增加白介素1（IL-1），肿瘤坏死因子（TNF-α）和裂解的caspase-3的表达，表现出对人神经胶质瘤干细胞（GSCs）的选择性细胞毒性，并诱导caspase-3依赖性外源性凋亡，同时破坏了GSC的无限扩散和自我更新能力。这一发现可能为选择性杀死人神经胶质瘤干细胞提供新型线索。