当前位置: X-MOL 学术Metallomics › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Embryonic exposure to an aqueous coal dust extract results in gene expression alterations associated with the development and function of connective tissue and the hematological system, immunological and inflammatory disease, and cancer in zebrafish†
Metallomics ( IF 3.4 ) Pub Date : 2018-02-15 00:00:00 , DOI: 10.1039/c7mt00300e
Karina Caballero-Gallardo 1, 2, 3, 4, 5 , Sara E. Wirbisky-Hershberger 6, 7, 8, 9 , Jesus Olivero-Verbel 1, 2, 3, 4, 5 , Jesus de la Rosa 10, 11, 12, 13, 14 , Jennifer L. Freeman 6, 7, 8, 9
Affiliation  

Coal mining is one of the economic activities with the greatest impact on environmental quality. At all stages contaminants are released as particulates such as coal dust. The first aim of this study was to obtain an aqueous coal dust extract and characterize its composition in terms of trace elements by ICP-MS. In addition, the developmental toxicity of the aqueous coal extract was evaluated using zebrafish (Danio rerio) after exposure to different concentrations (0–1000 ppm; μg mL−1) to establish acute toxicity, morphology and transcriptome changes. Trace elements within the aqueous coal dust extract present at the highest concentrations (>10 ppb) included Sr, Zn, Ba, As, Cu and Se. In addition, Cd and Pb were found in lower concentrations. No significant difference in mortality was observed (p > 0.05), but a delay in hatching was found at 0.1 and 1000 ppm (p < 0.05). No significant differences in morphological characteristics were observed in any of the treatment groups (p > 0.05). Transcriptomic results of zebrafish larvae revealed alterations in 77, 61 and 1376 genes in the 1, 10, and 100 ppm groups, respectively. Gene ontology analysis identified gene alterations associated with the development and function of connective tissue and the hematological system, as well as pathways associated with apoptosis, the cell cycle, transcription, and oxidative stress including the MAPK signaling pathway. In addition, altered genes were associated with cancer; connective tissue, muscular, and skeletal disorders; and immunological and inflammatory diseases. Overall, this is the first study to characterize gene expression alterations in response to developmental exposure to aqueous coal dust residue from coal mining with transcriptome results signifying functions and systems to target in future studies.

中文翻译:

胚胎暴露于含水煤尘提取物中会导致基因表达改变,与结缔组织和血液系统的发育和功能,免疫和炎性疾病以及斑马鱼的癌症有关

煤炭开采是对环境质量影响最大的经济活动之一。在所有阶段,污染物都会以颗粒形式释放,例如煤尘。这项研究的第一个目的是获得一种煤粉尘的水提物,并通过ICP-MS对其微量元素进行表征。此外,在暴露于不同浓度(0–1000 ppm;μgmL -1)后,使用斑马鱼(Danio rerio)评估了含水煤提取物的发育毒性。)建立急性毒性,形态和转录组变化。最高浓度(> 10 ppb)的煤粉尘提取物中的微量元素包括Sr,Zn,Ba,As,Cu和Se。另外,发现镉和铅的浓度较低。没有观察到死亡率的显着差异(p > 0.05),但是在0.1和1000 ppm处发现孵化延迟(p <0.05)。在任何治疗组中均未观察到形态学特征的显着差异(p> 0.05)。斑马鱼幼虫的转录组结果表明,分别在1、10和100 ppm组中有77、61和1376个基因发生了改变。基因本体分析确定了与结缔组织和血液系统的发育和功能有关的基因改变,以及与凋亡,细胞周期,转录和氧化应激有关的途径,包括MAPK信号通路。另外,基因改变与癌症有关。结缔组织,肌肉和骨骼疾病;以及免疫和炎性疾病。总体而言,这是第一项表征基因表达变化的研究,其转录过程结果表明了煤炭开采产生的含水煤尘残余物的发育暴露,其转录组结果表明了今后研究的功能和系统。
更新日期:2018-02-15
down
wechat
bug