Recent research revealed that tissue spray mass spectrometry enables rapid molecular profiling of biological tissues, which is of great importance for the search of disease biomarkers as well as for online surgery control. However, the payback for the high speed of analysis in tissue spray analysis is the generally lower chemical sensitivity compared with the traditional approach based on the offline chemical extraction and electrospray ionization mass spectrometry detection. In this study, high resolution mass spectrometry analysis of endometrium tissues of different localizations obtained using direct tissue spray mass spectrometry in positive ion mode is compared with the results of electrospray ionization analysis of lipid extracts. Identified features in both cases belong to three lipid classes: phosphatidylcholines, phosphoethanolamines, and sphingomyelins. Lipids coverage is validated by hydrophilic interaction liquid chromatography with mass spectrometry of lipid extracts. Multivariate analysis of data from both methods reveals satisfactory differentiation of eutopic and ectopic endometrium tissues. Overall, our results indicate that the chemical information provided by tissue spray ionization is sufficient to allow differentiation of endometrial tissues by localization with similar reliability but higher speed than in the traditional approach relying on offline extraction.

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最近的研究表明，组织喷雾质谱技术能够对生物组织进行快速的分子谱分析，这对于疾病生物标志物的搜索以及在线手术控制非常重要。但是，与基于离线化学提取和电喷雾电离质谱检测的传统方法相比，组织喷雾分析中高速分析的回报通常是较低的化学敏感性。在这项研究中，使用直接组织喷雾质谱法在正离子模式下获得的不同位置的子宫内膜组织的高分辨率质谱分析与脂质提取物的电喷雾电离分析结果进行了比较。在这两种情况下，已确定的特征均属于三类脂质：磷脂酰胆碱，磷酸乙醇胺，和鞘磷脂。通过亲水相互作用液相色谱与脂质提取物的质谱法验证脂质的覆盖率。来自这两种方法的数据的多变量分析显示，对位和异位子宫内膜组织具有令人满意的区别。总体而言，我们的结果表明，组织喷雾电离所提供的化学信息足以通过定位实现子宫内膜组织的分化，具有相似的可靠性，但速度高于传统方法（依赖于脱机提取）。

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