The successful regeneration of large volume bone defects necessitates the use of proangiogenic and resorbable scaffolding matrix. Impaired and slow ingrowth of host vasculature within implanted grafts greatly compromises its effective osseointegration. By addressing this, it is demonstrated that the use of copper doped bioactive glass functionalizes silk microfiber reinforcements to improve the physicochemical and osteoinductive properties of two silk scaffolding matrices (mulberry Bombyx mori and non‐mulberry Antheraea assama) employed in the study. The reinforced composite matrices increase the surface area and present an open porous biomimetic micromillieu favoring stem cell and endothelial cell migration within the matrix. Biochemical results indicate the stabilization of hypoxia‐inducible factor‐1α and expression of C‐X‐C chemokine receptor type‐4 in adipose derived human mesenchymal stem cells, which regulate the downstream proangiogenic signaling and endothelial cell homing, respectively. Osteoinduction, matrix turnover, and resorption effectiveness are favored better in the non‐mulberry silk matrices. The composite matrices significantly promote neo‐osseous tissue formation in volumetric femur defect in rabbits with periosteal restoration seen in the non‐mulberry silk composite matrices. Evidences of total resorption, enhanced vascular‐fibrous tissue ingrowth within the scaffold, vouch for the potential clinical translation of these developed composite silk matrices.

中文翻译：

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用于骨诱导，促血管生成和可吸收骨移植的多功能细胞指导性丝绸生物活性玻璃复合增强支架。

大体积骨缺损的成功再生需要使用促血管生成和可吸收的支架基质。植入的移植物中宿主脉管的受损和缓慢向内生长大大损害了其有效的骨整合。通过解决这个问题，证明了使用掺杂铜的生物活性玻璃可以使蚕丝微纤维增强材料功能化，从而改善两种蚕丝支架基质（桑蚕Bombyx mori和非桑蚕An蚕体）的物理化学和骨诱导特性。）从事这项研究。增强的复合基质增加了表面积，并呈现出开放的多孔仿生微球，有利于干细胞和内皮细胞在基质内的迁移。生化结果表明，缺氧诱导因子-1α的稳定和C-X-C趋化因子受体4型在脂肪来源的人间充质干细胞中的表达，分别调节下游促血管生成信号和内皮细胞归巢。在非桑蚕丝基质中，成骨诱导，基质转化和吸收效果更好。复合基质在非桑蚕丝复合基质中可明显促进具有骨膜修复的兔股骨缺损中新骨组织的形成。完全吸收的证据，